中国真菌学杂志2012，Vol.7Issue(6)：339-341,361,4.
利于甘露糖蛋白纯化的新生隐球菌培养方法研究
Fermentation culture of Cryptococcus neoformans benefiting for purification of mannoprotein
摘要
Abstract
Objective To study the possibility of fermentation culture of Cryptococcus neoformans and the advantages of fermentation culture supernatant used for purificating mannoprotein. Methods ConA Sepharose 4B affinity column was used to purificate mannoprotein from fermentation culture supernatant of CAP67. Results Up to milligram of mannoprotein was obtained from fermentation supernatants of Cryptococcus neoformans acapsular strain CAP67. Conclusion Fermentation method can be used to culture Cryptococcus neoformans , and the fermentation supernatants can be used for the purification of mannoprotein, which possessed the superiority of shorter time-consuming and higher yields compared to the traditional method.关键词
新生隐球菌/发酵/荚膜多糖/甘露糖蛋白/分离与纯化Key words
Cryptococcus neoformans / fermentation/ capsular polysaccharide/ mannoprotein/ isolation and purification分类
医药卫生引用本文复制引用
李平,谭宏月,钟彬,陈丽华,胡婵,朱红梅,温海..利于甘露糖蛋白纯化的新生隐球菌培养方法研究[J].中国真菌学杂志,2012,7(6):339-341,361,4.基金项目
国家自然科学基金(30870106,81071336) （30870106,81071336）
