中国肿瘤生物治疗杂志2012，Vol.19Issue(6)：599-603,5.DOI:10.3872/j.issn.1007-385X.2012.06.006
miRNA-34a对胶质瘤SHG-44细胞增殖和凋亡的影响
Effects of miRNA-34a on proliferation and apoptosis of glioma SHG-44 cells
摘要
Abstract
Objective: To investigate the expression levl of microRNA-34a (miR-34a) in human glioma tissues, and further explore the role of miR-34a on proliferation and apoptosis of glioma SHG-44 cells. Methods: Twenty glioma samples were collected from the Department of Neurosurgery, Affiliated Hospital of Qingdao Medical College (January 2007 to December 2010). The normal brain tissues were obtained from 5 patients with severe traumatic brain injury who required post-trauma surgery. The expression level of MiR-34a in glioma tissues was detected by real-time PCR. After transfection of miR-34a mimics into SHG-44 cells, the proliferation, cell cycle and apoptosis were measured by MTT and flow cytometry, respectively. Results: The expression level of miR-34a was lower in the glioma tissues compared with the normal brain tissues, and miR-34a expression level was lower in the glima tissues of phase Ⅲ/Ⅳ than in phase Ⅰ/Ⅱ. The cell proliferation inhibitory rate increased signficantly in the miR-34a transfected. group compared to the blank group ( [37. 24 ±5. 72] % vs [4. 19 ± 0. 63 ] % , P ＜ 0. 01) , the ratio of cells arrest at G, phase was significantly higher in the miR-34a mimics transfected group compared to the blank group ([61.78 ±2.01 ]% vs [50.91 ± 1. 19]% , P＜0.05), and the cell apoptosis in the miR-34a transfected group was significantly increased compared to the blank group ( [ 15. 28 ± 3. 65 ] % vs [ 2. 07 ± 0. 84 ] % , P ＜ 0. 01). Conclusion: MiR-34a was lowly expressed in human glioma tissues. MiR-34a can inhibit SHG-44 cell proliferation, thus inducing SHG-14 cell cycle arrest and promoting SHG-44 cell apoptosis.关键词
微小RNA/microRNA-34a/胶质瘤/SHG-44细胞/增殖/凋亡Key words
microRNA/ microRNA-34a/ glioma/ SHG-44 cell/ proliferation/ apoptosis分类
医药卫生引用本文复制引用
刘鹏,伦鹏,孟庆海..miRNA-34a对胶质瘤SHG-44细胞增殖和凋亡的影响[J].中国肿瘤生物治疗杂志,2012,19(6):599-603,5.基金项目
山东省自然科学基金重点项目资助(No.Y2006C02). （No.Y2006C02）
