中国肿瘤生物治疗杂志2012，Vol.19Issue(6)：604-608,5.DOI:10.3872/j.issn.1007-385X.2012.06.007
重组TNF-α慢病毒感染的脐血间质干细胞对胃癌移植瘤生长的抑制作用
Inhibitory effect of umbilical cord blood mesenchymal stem cells infected with reconstructed lentivirus-TNF-α on growth of gastric cancer transplantation tumors
摘要
Abstract
Objective: To study the inhibitory effect of umbilical cord blood mesenchymal stem cells (UCBMSCs) infected with reconstructed lentivirus-TNF-α on growth of gastric cancer transplantation tumors, based on UCBMSC as a TNF-α carrier. Methods: The human gastric cancer SGC-7901 cells were injected into nude mice subcutaneously groin. The model of transplanted SGC-7901 cells in nude mice was set up. The reconstructed lentivirus (Lv-TNF-α) and empty lentivirus (Lv-EGFP) were added to UCBMSC, and UCBMSC-TNF-α cells and control UCBMSC-EGFP cells were obtained. Tumor-bearing nude mice were separated into three groups randomly; a UCBMSC-TNF-a group, an UCBMSC-EGFP group and a NaCl group. The nude mice in these three groups were injected around the tumor with UCBMSC-TNF-a cells, UCBMSC-EGFP cells or NaCl. The transplanted gastric cancer volume and weight was observed; the expressions of TNF-α mRNA and protein in the three groups were determined by RT-PCR and ELISA; and the necrosis areas in the tumors were observed by H-E staining. Results: The transplantation tumor model was established in the nude mice successfully. The transplanted tumor average volume in the three groups were (0.51 ±0.27) , (0.64 ±0.36) and (1.21 ± 0.80) cm , and the transplanted tumor average volume in the UCBMSC-TNF-a group was minimum (F =3. 88, P ＜0. 05 ) ; The expressions of TVF-α mRNA in the three groups were (1. 92 ±0. 12) , ( 1. 21 ±0. 26) and (0. 81 ±0. 22) , and the expression of TNF-α mRNA in the UCBMSC-TNF-α group was maximum ( F = 54. 82 , P ＜ 0. 01) ; The expressions of TNF-α protein in the three groups were ( 148. 29 ± 3. 76) , (78. 22 ± 6. 24 ) and (42. 80 ± 3. 02 ) pg/ml, and the expression of TNF-α protein in the UCBMSC-TNF-α group was maximum ( F = 694. 54, P ＜ 0. 01 ) ; H-E stained biopsy revealed that the largest tumor necrosis was in the UCBMSC-TNF-α treatment group. Conclusion: Transgenic UCBMSCs can paracrine TNF-α., which has an inhibitory effect on gastric cancer.关键词
慢病毒/TNF-α/脐血间质干细胞/转基因治疗/胃癌Key words
lentivirus/ TNF-α/ umbilical cord blood mesenchymal stem cell/ transgenosis therapy/ gastric cancer分类
医药卫生引用本文复制引用
马贵亮,张学斌,朱新红,李杨,毛伟征..重组TNF-α慢病毒感染的脐血间质干细胞对胃癌移植瘤生长的抑制作用[J].中国肿瘤生物治疗杂志,2012,19(6):604-608,5.基金项目
国家自然科学基金资助项目(No.30772542). （No.30772542）
