中国肿瘤生物治疗杂志2012，Vol.19Issue(6)：615-620,6.DOI:10.3872/j.issn.1007-385X.2012.06.009
慢病毒介导RhoA基因沉默对卵巢癌细胞侵袭和迁移的影响
Effect of lentivirus-mediated silence of RhoA gene on invasion and migration of ovarian cancer cells
摘要
Abstract
Objective; To construct a ovary cancer cell line HO8910 with a stable knockdown of RhoA gene by lentivi-ral vectors; and to investigate the effect of RhoA gene on invasion and migration of HO8910 cells. Methods; Lenti-U6-shRhoA lentivirus targeting silence RhoA gene expression was constructed and infected into HO8910 cells. HO8910 cells stable knockdown RhoA gene expression were obtained by puromycin selection. The expression of RhoA mRNA in HO8910 cells was detected by real-time PCR; the proliferation of HO8910 cells was detected by MTT assay; and Transwell migration assay and Matrigel invasion assay were used to measure the effect of RhoA gene silence on invasion and migration of HO8910 cells. Results; The lentiviral vector Lenti-U6-shRhoA targeting RhoA gene was constructed successfully and a stable RhoA knockdown HO8910 cell line was established. The RhoA gene expression was efficiently suppressed by Lenti-U6-shRhoA with a expression rate of (30.7 ±5.4)% , compared with the control Lenti-U6-NC group (95.9 ±6.53)% ( P ＜0. 05 ). The knockdown of RhoA gene inhibited the survival rate of HO8910 cells ( [51. 16 ±7. 41 ] % vs [95. 67 ± 2. 14 ] % , P ＜ 0. 05 ) ; Compared with the Lenti-U6-NC group, the capabilities of the invasion (97 ± 12 vs 689 ± 23 , P ＜ 0. 05 ) , migration (31 ± 6 vs 84 ± 13 , P ＜ 0. 05 ) and adhesion rate ( [ 68. 16 ± 6. 53 ] % vs [ 98. 71 ± 4. 92 ] % , P ＜ 0.05) were also obviously suppressed in the RhoA stable knockdown HO8910 cell line. Conclusion; The lentivirus-mediated RhoA gene silence can suppress the invasion and migration of ovary cancer HO8910 cells.关键词
慢病毒/RNA干扰/RhoA基因/卵巢癌/侵袭/迁移Key words
lentivirus/ RNA interfence/ RhoA gene/ ovary cancer/ invasion/ migration分类
医药卫生引用本文复制引用
杨文娟,康佳丽,王小霞,刘启才,王冬昱,贾菡..慢病毒介导RhoA基因沉默对卵巢癌细胞侵袭和迁移的影响[J].中国肿瘤生物治疗杂志,2012,19(6):615-620,6.基金项目
广东省科技计划资助项目(No.2010B031600185). （No.2010B031600185）
