中国肿瘤生物治疗杂志2013，Vol.20Issue(1)：37-42,6.DOI:10.3872/j.issn.1007-385X.2013.01.006
线粒体融合蛋白-2基因增强人乳腺癌T47D细胞对小白菊内酯的敏感性
Mitofusin-2 gene enhances sensitivity of human breast cancer T47D cells to parthenolide
摘要
Abstract
Objective: To investigate the effect of mitofusin-2 (Mfn-2) gene expression on sensitivity of human breast cancer T47D cells to parthenolide. Methods: The expressions of Mfn-2 mRNA in various breast cancer cell lines (T47D, MDA-MB-231 , MCF-7 , MDA-MB435 and HCC38) were detected by real-time PCR. Plasmids pEGFP and pEGFP-Mfn-2 were transfected into human breast cancer T47D cells by Lipofectamine? 2000 in vitro. The expression levels of Mfn-2 mRNA and protein in T47D cells were detected by real-time PCR and Western blotting. MTT assay was used to detect the proliferation of T47D cells. The cell apoptotic rate and mitochondrial membrane potential of T47D cells were measured by flow cytometry. Results: Compared with that in normal breast cells, Mfn-2 mRNA was highly expressed in breast cancer HCC38 cell line, and lowly expressed in the other cell lines, such as T47D etc. After pEGFP-Mfn-2 transfection for 48 h, the expression levels of Mfn-2 mRNA and protein were significantly up-regulated in T47D cells. Compared with the pEGFP transfection group, the pEGFP-Mfn-2 transfection group showed a significant decrease in surivival rate of T47D cells under the treatment of parthenolide ( 50 mmol/L) ( [ 47. 93 ± 2. 21 ] % vs [ 56. 93 ± 2. 05 ] % , P ＜ 0. 05 ) . Flow cytometry results showed that the apoptotic rate of T47D cells under the treatment of 0. 05 mol/L parthenolide was significantly in-creased in pEGFP-Mfn-2 transfection group compared with that in pEGFP transfection group ([71. 2 ±2. 1] % vs [38. 8 ±2. 6] % , P ＜0. 05). However, the mitochondrial membrane potential was significanly decreased in the pEGFP-Mfn-2 transfection group ([1.6+0.1] % vs [5.0±0.5] % , P＜0.05). Conclusion: pEGFP-Mfn-2 transfection can enhance the sensitivity of T47D cells to parthenolide.关键词
乳腺癌/T47D细胞/线粒体融合蛋白-2/小白菊内酯/敏感性Key words
breast cancer/ T47D cell/ mitofusin-2/ parthenolide/ sensitivity分类
医药卫生引用本文复制引用
邱梅清,佟仲生,贾勇圣,刘晓东,陈悦..线粒体融合蛋白-2基因增强人乳腺癌T47D细胞对小白菊内酯的敏感性[J].中国肿瘤生物治疗杂志,2013,20(1):37-42,6.基金项目
天津市自然科学基金资助项目(No.10JCYBJC11500). （No.10JCYBJC11500）
