中国肿瘤生物治疗杂志2013，Vol.20Issue(1)：43-47,5.DOI:10.3872/j.issn.1007-385X.2013.01.007
TRAIL联合紫杉醇对人脑胶质瘤U87细胞的抑制效应及其可能的机制
Inhibitory effects of paclitaxel combined with TRAIL on human glioma U87 cells and the possible mechanism
摘要
Abstract
Objective: To investigate the inhibitory effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) combined with paclitaxel treatment on human glioma U87 cells and the possible mechanism. Methods: MTT assay was used to detect the proliferation inhibitory rates of U87 cells in the paclitaxel group, TRAIL group, and TRAIL/ paclitaxel combination group, and flow cytometry was used to detect the effects of different treatments on apoptosis of U87 cells. The expression levels of TRAIL death receptor (DR) 4, DR5, caspase-8 and caspase-3 in U87 cells after different treatments were measured by Western blotting. Results: MTT results showed the TRAIL or paclitaxel used alone demonstrated a favorable inhibitory effect on proliferation of U87 cells in a concentration-dependent manner. Combined application of TRAIL (500 ng/ml) and paclitaxel (0.5 μmol/L) showed a synergistic inhibitory effect on the proliferation of U87 cells with the coefficient of drug interaction ( CDI) being 0. 59. The proliferation inhibitory rate of U87 cells in the combination group was significantly higher than that in the TRAIL or paclitaxel used alone groups ([70.24 ±3. 68] % vs [ 27. 01 ± 2. 36 ] % , [21.31 ±4. 85] % , P ＜0. 01) . The apoptotic rate of U87 cells in the TRAIL/paclitaxel combination group was significantly higher than that in the control group, TRAIL group, or paclitaxel group ( [ 67. 67 ± 2.46 ] % vs [ 1. 80 ± 1. 13 ] % , [22. 13 ±2. 18] % , [ 35. 90 ± 2. 53 ] % , P ＜ 0. 01). The up-regulation expressions DR4,caspase-8 and caspase-3 in U87 cells was more obvious in TRAIL/paclitaxel combination treatment group than that in the TRAIL or paclitaxel groups ( P ＜ 0. 05 ) . However, no obvious change in DR5 expression was observed ( P ＞ 0. 05 ) . Conclusion: TRAIL combined with paclitaxel treatment can up-regulate DR4, caspase-8 and caspase-3 expressions, thereby inhibiting the proliferation and inducing the apoptosis of U87 cells.关键词
脑胶质瘤/U87细胞/肿瘤坏死因子相关凋亡配体/紫杉醇/凋亡Key words
glioma/ U87 cell/ TRAIL/ paclitaxel/ apoptosis分类
医药卫生引用本文复制引用
仇波,吴鹏飞,王勇,陶钧,欧绍武,王运杰..TRAIL联合紫杉醇对人脑胶质瘤U87细胞的抑制效应及其可能的机制[J].中国肿瘤生物治疗杂志,2013,20(1):43-47,5.基金项目
国家自然科学基金资助(No.81000565). （No.81000565）
