中国肿瘤生物治疗杂志2013，Vol.20Issue(1)：70-74,5.DOI:10.3872/j.issn.1007-385X.2013.01.012
腺病毒介导PTEN过表达抑制人脑胶质瘤U251细胞裸鼠移植瘤的生长
Adenovirus-mediated over-expression of PTEN inhibits the growth of human glioma U251 cell xenografts in nude mice
摘要
Abstract
Objective: To investigate the inhibitory effect of recombinant adenovirus ( Ad-PTEN-GFP) containing phosphatase and tensin homologue deleted on chromosome 10 (PTEN) gene on the growth of human glioma U251 cell xenografts in nude mice. Methods： Human glioma U251 cells were injected under the skin of the dorsum to establish a nude mouse glioma model. The tumor-bearing nude mice were randomly divided into three groups; Ad-PTEN-GFP group, Ad-GFP group ( empty vector group) and PBS group ( blank group). The growth of tumor xenografts in nude mice of the three groups was observed. The tumor volumes were measured, the tumor growth curves were drawn and the survival time of the nude mice bearing tumors was observed. The cell apoptosis of tumor cells was detected by TUNEL assay, and the expressions of PTEN and P65 proteins in tumor tissues were detected by immuno-histochemical assay. Results： Compared with the Ad-GFP group, the growth of glioma cell xenografts in the Ad-PTEN-GFP group was inhibited with tumor volume inhibition rate increasing significantly ([82.5 ±12. 7]% vs [7. 2±1.3]%, P ＜ 0. 05 ) , and the survival time of tumor-bearing nude mice was prolonged ( [ 103 ± 10 ] vs [58 ±8] d, P ＜0. 01 ] . TUNEL assay results showed that the apoptotic rate of glioma cells was significantly increased ( [46. 4 ± 8. 3 ] % vs [4. 6 ± 1. 0] % , P ＜0. 01 ]. Immunohistochemical assay results showed that the positive expression rate of PTEN protein was increased in the Ad-PTEN-GFP group compared with the Ad-GFP group (83.3% vs 0 , P ＜0.0l) , and the positive expression rate of P65 protein was decreased (16.7% vs 66. 7% , P ＜ 0.01). Conclusion; The growth of human glioma U251 cell xenografts in nude mice is suppressed after Ad-PTEN-GFP infection, which may be related with the down-regulation of P65 protein expression.关键词
胶质瘤/U251细胞/10号染色体同源丢失性磷酸酶-张力蛋白(PTEN)/P65/凋亡/重组腺病毒载体Key words
glioma/ U251 cell/ PTEN/ P65 / apoptosis/ recombinant adenovirus vector分类
医药卫生引用本文复制引用
郑克彬,刘海鹏,单小松,李志红,李春晖,方川..腺病毒介导PTEN过表达抑制人脑胶质瘤U251细胞裸鼠移植瘤的生长[J].中国肿瘤生物治疗杂志,2013,20(1):70-74,5.基金项目
河北省自然科学基金资助项目(No.2012201136) （No.2012201136）
河北大学医学学科专项资金资助项目(No.2012B2004) （No.2012B2004）
