肿瘤防治研究2012，Vol.39Issue(11)：1285-1288,4.DOI:10.3971/j.issn.1000-8578.2012.11.001
白花丹素对骨肉瘤细胞系U2OS的作用及其机制
Effect and Mechanism of Plumbagin in Human Osteosarcoma Cell Line U2OS
摘要
Abstract
Objective To explore the effect of plumbagin on the growth of osteosarcoma cell line U2OS and its possible underlying mechanisms. Methods Cell apoptosis of plumbagin against osteosarcoma was studied by CCK-8 assay;morphological changes was studied by HOECHST 33342 staining;the expression of p53 and MDM2 gene was studied by Western blot assay. Results CCK-8 assay showed that plumbagin had an obvious inhibition on osteosarcoma cell line in a dose-dependent manner; HOECHST 33342 staining showed that plumbagin inhibited the growth of osteosarcoma cell line U2OS through the promotion of apoptosis;Western blot assay showed that plumbagin played the role by changing the expression proportion of p53 and MDM2. Conclusion Plumbagin inhibited osteosarcoma cell line U2OS cells growth through cell apoptotic pathways.关键词
白花丹素/骨肉瘤细胞/凋亡/p53基因/MDM2基因Key words
Plumbagin/Osteosarcoma cells/ Apoptosis/p53 gene/MDM2 gene分类
医药卫生引用本文复制引用
田林强,陈安民,尹德龙,宫晨,任晔,郭风劲..白花丹素对骨肉瘤细胞系U2OS的作用及其机制[J].肿瘤防治研究,2012,39(11):1285-1288,4.基金项目
973课题资助项目(2002CB513100) （2002CB513100）
