肿瘤防治研究2012，Vol.39Issue(11)：1306-1310,5.DOI:10.3971/j.issn.1000-8578.2012.11.006
番茄红素诱导人结肠癌SW480细胞凋亡的作用机制
Mechanism of Lycopene Induced Cell Apoptosis on Human Colon Cancer SW480 Cells
摘要
Abstract
Objective To investigate the effect of Lycopene on the apoptosis of human colon cancer SW480 cells and the relevant molecular mechanism. Methods The anti-proliferative effect of a different concentration of Lycopene on SW480 cells was measured by MTT assay. Ratio of SW480 cells apoptosis was analyzed by flow cy-tometry. The levels of Bcl-2,Bax were detected by RT-PCR and Western blot. The expression levels of Caspase-9 and Caspase-3 were analyzed by Western blot. Results The MTT assay showed that Lycopene had potent anti-proliferative effect on SW480 cells in a time- and dose-dependent manner. The IC50 value of Lycopene was SW480 cells treated with 1. 0 μmol/L Lycopene for 24 hours. Flow cytometry analysis revealed that Lycopene increased the percentage of apoptotic SW480 cells. The expression level of Bcl-2 were decreased, while the expression levels of Bax,Caspase-9 and Caspase-3 were increased significantly in SW480 cells treated with different concentrations of Lycopene. Conclusion Lycopene could induce apoptosis of SW480 cells by decreasing the ratio of Bcl-2/Bax and increasing Caspase-9 and Caspase-3 expression.关键词
番茄红素/结肠癌/SW480细胞/细胞凋亡Key words
Lycopene/Colon cancer/SW480 cells/Cell apoptosis分类
医药卫生引用本文复制引用
赵其辉,邱青朝,胡波..番茄红素诱导人结肠癌SW480细胞凋亡的作用机制[J].肿瘤防治研究,2012,39(11):1306-1310,5.基金项目
湖南环境生物职业技术学院院长科研基金资助项目(Z08-02) （Z08-02）
