肿瘤防治研究2012，Vol.39Issue(11)：1317-1320,4.DOI:10.3971/j.issn.1000-8578.2012.11.009
溶瘤腺病毒Ad-MK联合外照射对膀胱癌细胞增殖的影响
Effect of Oncolytic Adenoviruses Ad-MK Combined with Irradiation on Bladder Cancer
摘要
Abstract
Objective To study the effect of oncolytic adenoviral Ad-MK alone or combined with or without irradiation on human transitional cell carcinoma cells EJ. Methods RT-PCR was employed to detect midkine and Adenovirus El a mRNA expression in EJ cells after infection with Ad-MK. The survival rations of the cells were performed by MTT. The effect of Ad-MK combined with radiation was performed by survival fraction. Virus yield of Ad-MK was determined by TCID50. Results The midkine mRNA and El a mRNA expressed in EJ cells. Ad-MK significantly inhibited cell proliferation in a dose- and time-dependent manner. The difference of survival fraction in combined group was statistically significant (P< 0.01). Cells treated with Ad-MK plus radiation produced a larger burst size. Conclusion The midkine mRNA is expressed in EJ cells and can selectively replication in them. Ad-MK significantly inhibited cell proliferation in a dose- and time-dependent manner. The cytostatic effects was superior to either virus or radiation treatment alone. Radiation can augment virus replication.关键词
溶瘤腺病毒/外照射/膀胱癌Key words
Oncolytic adenoviruses/Irradiation/Bladder cancer分类
医药卫生引用本文复制引用
张明,蔺强,石金苓,赵静,闫晓路..溶瘤腺病毒Ad-MK联合外照射对膀胱癌细胞增殖的影响[J].肿瘤防治研究,2012,39(11):1317-1320,4.基金项目
河北省医学科学重点指令性课题(20090029) （20090029）
