肿瘤防治研究2012，Vol.39Issue(12)：1407-1410,4.DOI:10.3971/j.issn.1000-8578.2012.12.001
EGCG对鼻咽癌细胞增殖、凋亡及E2F-1表达的影响
Effect of EGCG on Proliferation,Apoptosis and E2F-1 Expression of Nasopharyngeal Carcinoma Cell
摘要
Abstract
Objective To study the effects of epigallocatechin-3-gallate(EGCG)on proliferation and apoptosis of nasopharyngeal carcinoma cell and its mechanism. Methods The growth and proliferation of CNE-2Z cell was detected by CCK-8 assay,and apoptosis was assessed by AnnexinV/PI flow cytometry. The expressions of nuclear transcription factor E2F-1 and its regulatory protein CyclinD1 and p21 were detected by real-time quantitative PCR and Western blot. Results The proliferation of CNE-2Z cell was inhibited in a dose-dependent manner after treatment with EGCG, the apoptosis was induced as well. The expression of E2F-1 mRNA and protein in CNE-2Z cell were markedly suppressed by EGCG,and the level of its regulatory protein CyclinDl also decreased while p21 was increased significantly. Conclusion EGCG suppressed the proliferation and induced apoptosis in CNE-2Z cell by directly or indirectly down-regulating E2F-1 .which was regulated by CyclinDl and p21.关键词
表没食子儿茶素没食子酸酯/鼻咽癌/细胞凋亡/E2F-1Key words
(-)-Epigallocatechin-3-gallate/ Nasopharyngeal Carcinoma/Cell apoptosis/ E2F-1分类
医药卫生引用本文复制引用
李彬彬,黄培春,黄国良,何志巍..EGCG对鼻咽癌细胞增殖、凋亡及E2F-1表达的影响[J].肿瘤防治研究,2012,39(12):1407-1410,4.基金项目
广东省中医药管理局资助项目(81072059) （81072059）
