肿瘤防治研究2012，Vol.39Issue(12)：1469-1473,5.DOI:10.3971/j.issn.1000-8578.2012.12.015
上皮性卵巢癌组织中WWOX基因启动子区域CpG岛的甲基化状态及临床意义
Methylation State of WWOX Gene Promoter CpG Islands in Epithelial Ovarian Cancer and Its Clinical Significance
摘要
Abstract
Objective To evaluate the methylation status of CpG islands in WWOX gene promoter region of epithelial ovarian cancer, and to explore the relationship between methylation state of WWOX gene CpG island and clinicopathological indexes in epithelial ovarian cancer. Methods The methylation state of WWOX gene CpG island was evaluated by methylation specific polymerase chain reaction(MSP) in 48 patients with epithelial ovarian cancer, 18 patients with borderline epithelial ovarian tumors,26 patients with epithelial benign tumors,and 33 patients with normal ovarian tissues. Results The rates of CpG island methylation in WWOX gene promoter region in epithelial ovarian cancer tissues,borderline ovarian tumor tissues and benign ovarian tumor tissues were 43, 75% ,26. 32% and 3. 84% , respectively. Normal ovarian tissues were completely unmethylated in WWOX gene CpG island. The rate of CpG island methylation in epithelial ovarian cancer tissues was higher than that in other ovarian tissues,and these differences were found to be statistically significant (P<0. 01), The rate of CpG island methylation in WWOX gene promoter region in late-stage (stage Ⅲ , Ⅳ ) epithelial ovarian cancer tissues was higher than that in early-stage(stage Ⅰ , Ⅱ ) epithelial ovarian cancer tissues, and these differences were found to be statistically significant(P<0.05). Conclusion Epithelial ovarian cancer tissues show widespread CpG island methylation in WWOX gene promoter region,which may be explained as the important mechanism that leads to WWOX gene inactivation. A typical methylation of WWOX gene is germane to generation and progression of epithelial ovarian cancer, which makes it probably be an important indicator in epithelial ovarian cancer early diagnosis and prognosis evaluation.关键词
上皮性卵巢癌/WWOX基因/CpG岛/DNA甲基化Key words
Epithelial ovarian cancer/ WWOX gene/CpG island/DNA methylation分类
医药卫生引用本文复制引用
闫洪超,孙洁芸,陆晓媛,韩秋峪,魏敏..上皮性卵巢癌组织中WWOX基因启动子区域CpG岛的甲基化状态及临床意义[J].肿瘤防治研究,2012,39(12):1469-1473,5.基金项目
徐州市科技发展基金资助项目(XF10C086) （XF10C086）
