中山大学学报（医学科学版）2013，Vol.34Issue(1)：6-10,5.
1-磷酸神经鞘氨醇对大鼠骨骼肌成肌细胞分化的影响
Effects of Sphingosine 1-phosphate on Differentiation of Skeletal Myoblast Cell in Rats
摘要
Abstract
[Objective] To explore the effects and mechanism of sphingosine 1-phosphate (S1P) on differentiation of skeletal myoblast cells into mature myocytes and consequent formation of myotubes in rats. [Methods] Primary skeletal myoblast cells in rats were separated and cultured, here we described the roles of S1P in the regulation of differentiation by observing alteration of their appearance under phase contrast microscope and detected for the expression change of muscle-associated gene- myosine and myogenin by immunocytochemistry. Sphingosine kinase inhibitors were used to during S1P on differentiation of myoblast cells. [Results] S1P remarkably promoted myotude formation of myoblasts. Induced with S1P for 2-3 days, myotubes started to form. Later on, more and more myotubes appeared, and at the peak on 6-7 days. S1P induced myogenin positive nucleis higher with concentration-dependent form compare with control (P ＜ 0.01). But SphK inhibitors reduce S1P effect on differentiation of myoblast cells (P ＜ 0.01). [Conclusion] S1P may through sphingosine kinase promote differentiation of skeletal myoblast cells into mature myocytes and formation of myotube.关键词
1-磷酸神经鞘氨醇/磷酸神经鞘氨醇激酶/骨骼肌成肌细胞/分化/肌管Key words
S1P/ SphK/ skeletal myoblast cells/ differentiation/ myotube分类
生物科学引用本文复制引用
于欢,郑美蓉,余敬谋,陈培良,李卫东..1-磷酸神经鞘氨醇对大鼠骨骼肌成肌细胞分化的影响[J].中山大学学报（医学科学版）,2013,34(1):6-10,5.基金项目
国家青年科学基金(81000075) （81000075）
江西省教育厅重点科研项目GJJ11694) （）
