中山大学学报（医学科学版）2013，Vol.34Issue(1)：28-35,8.
EBV LMP2A对胃癌细胞生物学特性的影响
Effects of EBV LMP2A on Biological Characters of Gastric Cancer Cells
摘要
Abstract
[Objective] To investigate the effects of EBV LMP2A on biological characters of gastric cancer cells. [Methods] Human gastric cancer cell line SGC7901 was transfected with a retroviral vector containing the LMP2A gene. The expression of LMP2A gene mRNA was detected by QRT-PCR. The cell proliferation was detected by CCK8 assay. Cell apoptosis were analyzed by flow cytometry. The migration of cell was measured with wound healing assay and Transwell test. Cell tumorigenicity was measured with colony formation assay.[Results] The LMP2A gene-transfected gastric cancer cells grew vigorously and rapidly, had less apoptotic cells, formed more colonies and up-regulated the ability of migration. [Conclusion] The LMP2A gene can significantly enhance the malignancy of gastric cancer cells.关键词
EBV/LMP2A/转染/胃癌/SGC7901/凋亡/生物学特性Key words
EBV/ LMP2A/ transfection/ gastric carcinoma/ SGC7901/ apoptosis/ biological characters分类
医药卫生引用本文复制引用
蒋叶,冯国开,陈健宁,张娜娜,曾木圣,邵春奎..EBV LMP2A对胃癌细胞生物学特性的影响[J].中山大学学报（医学科学版）,2013,34(1):28-35,8.基金项目
国家自然科学基金(81071893,3067240,81272553) （81071893,3067240,81272553）
广东省自然科学基金(S2012020010898) （S2012020010898）
广州市科技计划项目(2011J4100106) （2011J4100106）
