作物学报2012，Vol.38Issue(12)：2178-2184,7.DOI:10.3724/SP.J.1006.2012.02178
兼抗全蚀病和白粉病小麦新种质的创制与鉴定
Development and Characterization of Wheat Lines with Resistance to Take-All and Powdery Mildew Diseases
摘要
Abstract
"Take-all", primarily caused by Gaeumannomyces graminis var. Tritici (Ggt), and powdery mildew, mainly caused by Blumeria graminis f. Sp. Tritici (Bgt), are important diseases of wheat (Triticum aestivum L.) worldwide. The wheat cultivar Yangmai 18 carrying a powdery mildew resistance gene Pm21, shows broad-spectrum resistance to powdery mildew. We have isolated a lipid transfer protein gene TaLTPS from wheat. To study the role of TaLTP5 in wheat defense responses to the major pathogens of take-all, we introduced this gene into Yangmai 18 via bombarding the particle containing the TaLTPS expressing vector pA25-TaLTP5. The TaLTPS transgenic wheat plants from T0 to T3 generations were subjected to PCR, Southern blot, RT-PCR, and Q-RT-PCR analyses. We also evaluated the disease resistances of the TaLTPS transgenic plants against inoculating Ggt and Bgt. The PCR and Southern blotting results showed that the alien TaLTP5 was transferred and integrated into the genomes of three transgenic wheat lines, and inherited stably in the transgenic wheat lines. The RT-PCR and Q-RT-PCR results indicated that the introduced TaLTPS was over-expressed in transgenic wheat lines, which showed significantly-enhanced resistance to take-all, suggesting that TaLTPS gene is involved in the defense response to Ggt infection. In addition, the resistance of transgenic lines to powdery mildew was not influenced by the introduced gene TaLTPS. Thus, TaLTPS transgenic wheat Yangmai 18 exhibits resistance to both "Take-all" and powdery mildew.关键词
小麦脂质转移蛋白/TaLTP5/转基因小麦/全蚀病/白粉病/抗性Key words
Wheat lipid transfer protein/ TaLTP5/ Transgenic wheat/ Take-all/ Powdery mildew/ Resistance引用本文复制引用
祝秀亮,李钊,杜丽璞,徐惠君,杨丽华,庄洪涛,马翎健,张增艳..兼抗全蚀病和白粉病小麦新种质的创制与鉴定[J].作物学报,2012,38(12):2178-2184,7.基金项目
本研究由国家转基因生物新品种培育重大科技专项(2011ZX08002-001和2009ZX08002-006B)资助. （2011ZX08002-001和2009ZX08002-006B）
