作物学报2012，Vol.38Issue(12)：2185-2191,7.DOI:10.3724/SP.J.1006.2012.02185
甘蓝型黄籽油菜自交系转化体系的建立
Establishment of Transformation System Using Inbred Line of Yellow-Seeded Brassica napus
摘要
Abstract
In this study, we established a transformation system using an inbred line of yellow-seeded Brassica napus. Hypocotyl explants precultured for 5-7 d on Murashige and Skoog medium containing 20μmol L-1 acetosyringone were cocultured with Agrobacterium tumefaciens strain LBA4404 (pCNR) for 63-69 hours. The plasmid pCNR was constructed by inserting △6-fatty acid desaturase gene from Rhizopus stolonifer into plant high-efficient expression vector pCAMBIA2301G. Kanamycin-tolerant shoots were regenerated on shoot induction medium for three months after Agrobacterium inoculation. The average transformation efficiency was about 1.3% under optimal conditions. Results from GUS assay and PCR analysis of transformed plants indicated that the introduced gene was integrated into B. Napus genomes. The result of Southern blot revealed that those transformants carried one or two copies of the goal gene. The fatty acids of the transgenic plant seeds were analyzed by GC, and the γ-linolenic content was 8.2%.关键词
甘蓝型油菜/△6-脂肪酸脱饱和酶/转化Key words
Brassica napus/ △6-fatty acid desaturase/ Transformation引用本文复制引用
林呐,刘列钊,殷家明,王瑞,柴友荣,李加纳..甘蓝型黄籽油菜自交系转化体系的建立[J].作物学报,2012,38(12):2185-2191,7.基金项目
本研究由高等学校学科创新工程计划"111"项目(B12006)和国家自然科学基金项目(31071450)资助. （B12006）
