作物学报2012，Vol.38Issue(12)：2192-2197,6.DOI:10.3724/SP.J.1006.2012.02192
黑龙江省稻瘟病菌无毒基因分析及抗病种质资源筛选
Analyzing Avirulence Genes of Magnaporthe oryzae from Heilongjiang Province and Screening Rice Germplasm with Resistance to Blast Fungus
摘要
Abstract
The avirulence genes from 177 single spore strains of Magnaporthe grisea isolated from different rice varieties in Heilongjiang Province were detected by specific primers. The results indicated that Avr-pita, ACE1, Avr-pia, Avr-pit, Avr1-co39, Avr-pik, and Avr-pizt could be detected in the strains from different rice-growing regions, and the amplification frequencies of avirurence genes were associated with the geographical orgin and rice variety, ACE1 and Avr1-co39 showed the highest and lowest frequency, that was 61.6% and 31.6%, respectively. Moreover, 20 monogenic lines with known blast resistance genes from International Rice Research Institute (IRRI) were used to analyze the genes which were valuable in breeding program in Heilongjiang Province by artificial inoculation. The resistance gene Pi-9 had the broadest resistance spectrum to the isolates from six regions.关键词
稻瘟病菌/无毒基因/毒力频率/抗病性Key words
Magnaporthe grisea/ Avirulence gene/ Virulence frequency/ Resistance引用本文复制引用
李祥晓,王倩,罗生香,何云霞,朱苓华,周永力,黎志康..黑龙江省稻瘟病菌无毒基因分析及抗病种质资源筛选[J].作物学报,2012,38(12):2192-2197,6.基金项目
本研究由引进国际先进农业科学技术计划(948计划)项目(2006-G51)和比尔-盖茨基金项目资助. （948计划）
