作物学报2012，Vol.38Issue(12)：2206-2216,11.DOI:10.3724/SP.J.1006.2012.02206
利用与大豆灰斑病抗性基因连锁的SSR标记构建大豆品种(系)的分子身份证
Establishment of Molecular ID of Soybean Varieties (Lines) Using SSR Markers Linked to Resistance Genes against Cercospora sojina
摘要
Abstract
One hundred and three soybean varieties (lines) developed by 29 breeding units from six accumulated temperature zones in Heilongjiang were investigated. The resistances of 103 soybean varieties (lines) were identified with three races of Cercospora sonija. Eighty-six polymorphic alleles were detected with 19 SSR markers, for each primer, two to six allele variations were detected in all varieties (lines), with an average of 4.42. The diversity index of the 19 SSR loci ranged from 0.198 to 0.751 with an average of 0.606. The results of calculating specific index and genetic similarity among varieties (lines) showed that specific index of varieties (lines) ranged from 46.592 to 481.541, and the average was 87.415. According to fragment size of allele variation, the data calculated from the PAGE bands were analyzed by the software ID Analysis 1.0. Seven markers (Satt565, Satt547, Satt431, Sct_186, SOYGPATR, Satt244, and Sat_151) linked to the gene controlling resistance to Cercospora sojina were used to identify all 103 soybean lines, so that a set of molecular ID of soybean varieties (lines) was established.关键词
大豆灰斑病/生理小种/SSR/分子身份证Key words
Soybean frogeye leaf spot/ Physiological race/ SSR/ Molecular ID引用本文复制引用
丁俊杰,姜翠兰,顾鑫,杨晓贺,赵海红,申宏波,仕相林,刘春燕,胡国华..利用与大豆灰斑病抗性基因连锁的SSR标记构建大豆品种(系)的分子身份证[J].作物学报,2012,38(12):2206-2216,11.基金项目
本研究由黑龙江省自然科学基金项目(C200936),国家公益性行业(农业)科研专项(201103016-03A2)和国家现代农业产业技术体系建设佳木斯综合试验站项目资助. （C200936）
