作物学报2012，Vol.38Issue(12)：2297-2305,9.DOI:10.3724/SP.J.1006.2012.02297
大豆7S球蛋白α'亚基缺失及(α'+α)亚基双缺失品系的回交转育
Development of Soybean Lines with a'-Subunit or (α'+α)-Subunits Deficiency in 7S Globulin by Backcrossing
摘要
Abstract
The α'- and a-subunits of 7S globulin, the components of soybean storage protein as well as the main allergens, are important factors affecting the nutritional and processing quality of soybean. It is currently focused to improve the quality of soybean variety by reducing the contents of α' and a-subunits in soybean breeding. In the experiment, we successfully recombined the character of allergenic proteins deficiency into Chinese soybean, using a Japanese variety "Ri B" with (α'+α)-subunits deficiency of 7S globulin (β-conglycinin) as the donor parent, and soybean variety Dongnong 47 with high oil content, widely planted in Heilongjiang Province of China, as the recurrent parent. The results showed that the plants of α'-subunit-deficient lines (Cc) and (α'+α)-subunit-deficient lines (Cd) could normally grow, set seeds, and stably inherited. The average values of the yield traits in the two lines (Cc, Cd) increased compared with those in the recurrent parent Dongnong 47. The average protein contents in the two lines were higher than those in the parents. The protein content reached to 46.7% in the Cd lines. The average oil content in Cc and Cd lines was ranged between that of both parents and a bit more than that of "Ri B". Fat content in part lines was 20% more than that in Cc and Cd lines, respectively. Total Amino acid contents were increased by introducing α'-subunit and (α'+α)-subunit, amount of argin-ine (Arg) and asparagine (Asp) in two lines markedly increased. The sulfur-containing amino acid (Met and Cys) contents and the total amino acid content in Cd lines were 0.11% and 0.56% more than those in Dongnong 47, respectively. So the recombined lines have a great potential to enhance food safety and quality, and could be used in soybean breeding for quality.关键词
大豆/致敏蛋白缺失/高含硫氨基酸品系/回交选育Key words
Soybean (Glycine max [L.] Merr.)/ Deficiency of allergenic proteins/ High sulfur amino acids lines/ Backcrossing引用本文复制引用
宋波,蓝岚,田福东,拓云,白月,姜自芹,申丽威,李文滨,刘珊珊..大豆7S球蛋白α'亚基缺失及(α'+α)亚基双缺失品系的回交转育[J].作物学报,2012,38(12):2297-2305,9.基金项目
本研究由国家自然科学基金项目(31071440,30800625),黑龙江省普通高等学校青年骨干支持计划项目(1155G12),东北农业大学博士启动基金(2009RC47)和国家转基因生物新品种培育重大专项(2011ZX08004-004-006-002)资助. （31071440,30800625）
