作物学报2012，Vol.38Issue(12)：2312-2319,8.DOI:10.3724/SP.J.1006.2012.02312
应用IMP标记分析30份烟草品种的遗传关系
Genetic Diversity of 30 Tobacco Varieties Analyzed by IMP Markers
摘要
Abstract
Miniature Inverted repeat Transposable Element (MITEs) is a high copy number transposon family in plants. Molecular markers based on closely located MITEs are termed Inter MITE polymorphisms or IMP. We conducted the genetic distance evaluation and cluster analyses of 30 tobacco varieties using IMP markers. Among 133 primers screened, 32 primers had steady polymorphism bands. A total of 185 polymorphic bands were detected out of 30 varieties, with 5.78 polymorphism bands per IMP primer. The genetic distance among cultivars ranged from 0.02 to 0.81, with an average of 0.33. According to cluster analyses, 30 tobacco varieties could be classified into three groups. Group Ⅰ included sun-cured tobacco, oriental tobacco and flue-cured tobacco of northern China; group Ⅱ included NC series from USA, Coker series and Yunyan series from China; group Ⅲ included two burley tobacco cultivars. The cluster analysis result closely matched the classic relationship among the cultivars. The result showed that the IMP markers, successfully amplified in tobacco, generated fairly polymorphism and could be applied in generic diversity analysis. Thus, IMP markers establish a foundation for Tobacco genetic study and marker-assisted selection. The genetic diversity is very low among 30 tobacco varieties investigated in this study, indicating that a strategy to enrich tobacco germplasm for further breeding should be developed.关键词
IMP分子标记/烟草/遗传关系Key words
IMP molecular marker/ Tobacco/ Genetic diversity引用本文复制引用
谢贺,高玉龙,宋中邦,桂毅杰,樊龙江,肖炳光,卢秀萍..应用IMP标记分析30份烟草品种的遗传关系[J].作物学报,2012,38(12):2312-2319,8.基金项目
本研究由云南省科技厅项目(2008CD194)和云南省烟草公司项目(08A05)资助. （2008CD194）
