植物学报2012，Vol.47Issue(6)：581-593,13.DOI:10.3724/SP.J.1259.2012.00581
小麦叶绿体蛋白质编码基因RNA编辑位点的测定及与返白现象的关系
RNA Editing Sites in Chloroplast Protein-coding Genes in Leaf White Mutant of Triticum aestivum
摘要
Abstract
RNA editing is a post-transcriptional process and mainly occurs in higher plant organelles. Lacking RNA editing in some plants can cause albino or yellow leaves. We investigated RNA editing in 14 protein-coding genes from the chloroplast genome of Triticum aestivum Aibian 1 and the wheat leaf-stage albino mutant FA85. We found 26 editing sites in these genes. The two plants significantly differed in editing efficiency of 5 partial editing events. The 5 sites include the phosphoenylpyruvate (PEP) genes rpoC2, rpoA and rpoB, and 3 of them are predicted to change the secondary protein structure. We further analyzed the transcriptional pattern of chloroplast genes that depend on PEP, on nuclear export protein (NEP) and on both PEP and NEP. The mRNA levels of chloroplast genes, except for psbA and clpP, were lower in the mutant than wild-type leaves. The wheat leaf-stage albino phenotype may be attributed to a decrease in efficiency of the 5 editing sites and alteration of transcript level of some chloroplast genes.关键词
叶绿体/返白系/RNA编辑/小麦Key words
chloroplast/leaf white mutant/RNA editing/Triticum aestivum引用本文复制引用
邓李坤,李妍,俞嘉宁..小麦叶绿体蛋白质编码基因RNA编辑位点的测定及与返白现象的关系[J].植物学报,2012,47(6):581-593,13.基金项目
国家基础研究发展计划(No.2010CB126006)和陕西省农业攻关项目(No.2011K02-09) （No.2010CB126006）
