植物学报2012，Vol.47Issue(6)：594-601,8.DOI:10.3724/SP.J.1259.2012.00594
基于CSSL的高密度物理图谱定位水稻分蘖角度QTL
Identification of Tiller Angle Quantitative Trait Loci Based on Chromosome Segment Substituted Lines and High-density Physical Map in Rice
摘要
Abstract
We developed a 9311/Nipponbare physical map with 250 simple sequence repeat/sequence-tagged site markers by adding markers on the original genetic map. We screened 119 chromosome segment substituted lines and obtained 10 lines with significantly different tiller angle trait as compared with the recipient 9311 (P≤0.001). From this physical map, we detected quantitative trait loci (QTL) using the substitution map strategy. We identified 5 tiller-angle QTL, with qTA11 showing a positive effect and derived from the 9311 allele. Additional 4 QTL had a negative effect and were all derived from Nipponbare alleles. qTA6-1 and qTA6-2 were located on chromosome 6 at 3.55 Mb between RM253 and RM527 and at 1.65 Mb between RM3139 and RM494, respectively; qTA9 was located on chromosome 9 at 3.40 Mb between RM257 and RM189; qTA10 was located on chromosome 10 within 2.10 Mb between RM222 and S10-1; qTA11 was located on chromosome 11 within 3.30 Mb between RM1761 and RM4504. These results provide useful information for fine mapping new QTL of tiller angle and for rice breeding by plant type.关键词
染色体片段置换系/QTL/水稻/代换作图/分蘖角度Key words
chromosome segment substituted line/quantitative trait loci/rice/substitution mapping/tiller angle引用本文复制引用
赵春芳,周丽慧,于新,赵庆勇,陈涛,姚姝,张亚东,朱镇,王才林..基于CSSL的高密度物理图谱定位水稻分蘖角度QTL[J].植物学报,2012,47(6):594-601,8.基金项目
现代农业产业技术体系建设专项(No.CARS-01-47)和江苏省农业科技自主创新基金(No.CX(12)1003) （No.CARS-01-47）
