植物学报2012，Vol.47Issue(6)：615-624,10.DOI:10.3724/SP.J.1259.2012.00615
盐胁迫对不同品系杨树幼苗生长、细胞超微结构和离子稳态的影响
Effect of NaCl Stress on Growth, Cell Ultrastructure and Ion Homeostasis in Poplar Seedlings
摘要
Abstract
We examined the effect of salt stress on seedling growth, cell morphologic features and ultrastructure, as well as ion uptake and distribution for poplar seedlings of Nanyang 1 and 2. Under low salt stress (75 mmol·L-1NaCI), the growth inhibition was greater for Nanyang 1 than 2. Under high salt stress (150 mmol·L-1NaCI), growth inhibition did not differ between the 2 poplar strains. Low salinity produced greater damage to cell morphologic features and ultrastructure for Nanyang 1 than 2. In Nanyang 1, chloroplasts were bent and separated from the cell membrane, and the starch granule size was smaller and even absent. However, under high salinity, the damage was visible in both poplars. X-ray microanalysis revealed higher Na+ content in root cells of Nanyang 1 than 2 with salt stress, especially low stress. Under salt stress, in a new branch, Na+ content in epidermis, cortex and xylem cells was higher for Nanyang 1 than 2. In roots and new branches, Na+distributed first in epidermal and cortical cells of both poplars, which indicated that roots and new branches had strong Na+ retention; nevertheless, we observed slight or no significant Na+ increase in both poplar leaves. Under salt stress, in leaves, Na+ content in upper epidermis, palisade and spongy cells was higher for Nanyang 1 than 2, and except for upper epidermis cells, the Mg2+ content in the other cells was higher for Nanyang 2 than 1. In general, as compared with Nanyang 1, Nanyang 2 could maintain lower Na+ content in all root cells; the epidermis, cortex and xylem cells of new branches; the upper epidermis, palisade, and spongy cells of leaves; and palisade and spongy cells. Na+ flow to branches and leaves was lower in Nanyang 2 than 1. Thus, ionic homeostasis ability was greater in Nanyang 2 than 1 and may explain why Nanyang 2 has higher salt resistance than Nanyang 1.关键词
细胞形态/细胞超微结构/离子吸收和分配/NaCl胁迫/杨树幼苗Key words
cell morphology/cell ultrastructure/ion uptake and distribution/NaCl stress/poplar seedlings引用本文复制引用
武传兰,王长海,梁明祥,刘兆普,姜超强,郑青松..盐胁迫对不同品系杨树幼苗生长、细胞超微结构和离子稳态的影响[J].植物学报,2012,47(6):615-624,10.基金项目
公益性行业(农业)科研专项(No.200903001-05)和国家支撑项目(No.2011BAD13B09) （农业）
