植物学报2013，Vol.48Issue(1)：65-71,7.DOI:10.3724/SP.J.1259.2013.00065
近红外漫反射光谱法快速测定药用植物淫羊藿总黄酮含量
Rapid Determination of Total Flavonoids in a Medicinal Plant Epimedium by Near-infrared Reflectance Spectroscopy
摘要
Abstract
Epimedium is a famous medicinal plant in China. Total flavonoids in Epimedium exhibit a wide variety of bio-activities. We developed an analytical method based on near-infrared reflectance spectroscopy for determining total flavonoids in Epimedium and measured the content of total flavonoids in 7 Epimedium species. The best calibration model was under the following conditions: 2nd derivative, principal components analysis, weighted multiplicative scatter correction and modified partial least squares regression. The coefficient of determination for calibration, standard error of cross-validation and coefficient of determination for cross-validation of prediction equation of calibration set were 0.985 5, 0.023 0 and 0.044 3, respectively. The coefficient of determination, standard error of performance and bias of prediction equation of validation set were 0.965 1, 0.018 and 0.006, respectively. These results confirmed the good predictability of the model. The analytical method can be efficiently used for rapid quantification of total flavonoids in Epimedium.关键词
淫羊藿/近红外漫反射光谱/定量分析方法/总黄酮Key words
Epimedium/near-infrared reflectance spectroscopy/quantitatively analytical method/total flavonoids引用本文复制引用
薛耀碧,张华峰,杨晓华,牛丽丽,张翔,刘冬,李建科..近红外漫反射光谱法快速测定药用植物淫羊藿总黄酮含量[J].植物学报,2013,48(1):65-71,7.基金项目
中央高校基本科研业务费专项资金(No.GK200902043)和大学生创新性实验计划(No.CX11095) （No.GK200902043）
