植物研究2012，Vol.32Issue(6)：695-700,6.
新疆雪莲DFR基因的分离及同源遗传转化
Isolation and Homologous Genetic Transformation of DFR in Saussurea involucrata Kar.et Kir.
摘要
Abstract
The dihydroflavonol-4-reductase (DFR) is an important enzyme in plant secondary metabolites and catalyzes the key step in the biosynthesis of anthocyanins. DFR gene ( GenBank Accession JN092126) was cloned from Saussurea involucrata Kar. et Kir. by RT-PCR and RACE. The cDNA sequence of DFR was consisted of 1 029 bp open reading frame ( ORF) encoding 343 amino acid, the deduced DFR protein has high homolo-gy with S. medusa (92% identity). Homology analysis showed that deduced DFR protein has the special domain regioselectivity towards NADPH. DFR gene of S. involucrata was under the control of the cauliflower mosaic virus (CaMV) 35S promoter, homologous transformation was conducted using an Agrobacter-ium rihizogenes-mediated transformation system. The results of UV spectrophotometry showed that Sinvolucrata callus after suspension culture had an obviously higher average content of total flavonoids than non-transgenic callus. This study will improve the products of medicinal compositions and realize the synthesis of anthocyanins in 5. involucrata.关键词
新疆雪莲/DFR/同源转化/总黄酮Key words
Saussurea ircwlucrato/ dihydroflavonol-4-reductase /homoosis/flavonoids分类
农业科技引用本文复制引用
覃建兵,唐亚萍,曾卫军..新疆雪莲DFR基因的分离及同源遗传转化[J].植物研究,2012,32(6):695-700,6.基金项目
国家自然科学基金项目(30960092和30960044) （30960092和30960044）
