植物研究2012，Vol.32Issue(6)：765-769,5.
响应面优化纤维素酶辅助提取木豆叶总黄酮工艺
Optimization of Cellulase-assisted Extraction Process of Total Flavonoids from Pigeon Pea Leaves Using Response Surface Methodology
摘要
Abstract
Response surface methodology (RSM) was used to optimize the cellulase-assisted extraction process of total flavonoids (TF) from pigeon pea leaves. The amount of enzyme, enzymatic hydrolysis temperature, time and pH were selected as influencing factors and TF yield as the response value based on single factor trials. The experiment mathematical model was established according to Box-Behnken central composite experiment design and was analyzed by RSM. The results showed the optimal extraction conditions were as follows: enzyme amount 8. 65 mg, extraction temperature 33. 881 , extraction time 2. 02 h and extraction pH 5. 02. The predicted value of TF yield was 4. 86% under the optimum conditions, and the experimental value was 4. 88% . The optimal extraction conditions were stable and reliable, and would provide experimental basis for the future development of TF in pigeon pea leaves.关键词
木豆/总黄酮/纤维素酶/响应面Key words
pigeon pea/total flavonoids/cellulase/response surface methodology分类
农业科技引用本文复制引用
王秋雪,顾成波,付丽楠,李旺,袁肖寒,祖元刚,付玉杰..响应面优化纤维素酶辅助提取木豆叶总黄酮工艺[J].植物研究,2012,32(6):765-769,5.基金项目
哈尔滨市科技创新人才专项资金(2011RFQXS074)和中央高校基本科研业务费专项资金资助(DL09BA20) （2011RFQXS074）
