植物研究2013，Vol.33Issue(1)：91-97,7.DOI:10.7525/j.issn.1673-5102.2013.01.015
3种不同油桐种仁蛋白质提取方法比较研究
Comparison of Three Protein Extraction Methods for Tung trees (Vernicia fordii) Seed
摘要
Abstract
An effective protein extraction method is crucial to separate the proteins by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE).Tung tree (Vernicia fordii) seed,which is used for producing the tung oil,contains large amounts of compounds which can interfere with the extraction of proteins,and can even affect the resolution of 2D-PAGE images.In the present study,we developed a suitable protein extraction method from three tested methods,including trichloroacetic acid (TCA) -acetone ( Method A),phenol ( Method B),TCA-acetone combined with phenol ( Method C ).The results showed that the protein concentration of samples by Method C was high and it achieved 8.1 μg/μl.Method C has a strong capability to extract the high-molecularweight and the low-molecular-weight proteins from tung tree seeds.In addition,the 2D-PAGE image resolution of Method C was also better than the other two methods.Thus,Method C was used to extract proteins,and it would lay the foundation for proteomic analysis on the tung tree seeds.关键词
油桐种仁/蛋白质提取/双向电泳Key words
Tung tree seeds/protein extraction/two-dimensional polyacrylamide gel electrophoresis(2D-PAGE)分类
农业科技引用本文复制引用
占志勇,汪阳东,陈益存,韩小娇,崔琴琴..3种不同油桐种仁蛋白质提取方法比较研究[J].植物研究,2013,33(1):91-97,7.基金项目
Supported by the Financial Support of the State Forestry Administration,P.R.China (Grant No.20090423) （Grant No.20090423）
