中国中西医结合杂志2013，Vol.33Issue(1)：35-39,5.
载脂蛋白E基因多态性对血脂康胶囊抑制炎症作用的影响
Effects of ApoE Gene Polymorphism on Anti-inflammatory Action of Xuezhikang Capsule
摘要
Abstract
Objective To study the effects of ApoE gene polymorphism on anti-inflammatory action of Xuezhikang Capsule. Methods One hundred and two patients with hyperlipidemia (as the treated group)and one hundred healthy volunteers (as the control group)were enrolled in the case-control study. Total DNA of the peripheral blood was extracted and ApoE genotypes were determined by PCR sequence analysis. The serum levels of tumor necrosis factor alpha (TNF-α), inter leu kin 6 (IL-6), and high sensitivity C reactive protein (hs-CRP)were measured in all subjects. The changes of TNF-α, IL-6, and hs-CRP were detected before and after 6-week Xuezhikang Capsule treatment, thus analyzing the correlation between ApoE gene polymorphism and changes of each inflammatory factor. Results The frequency of E3/3 genotype was 86% (86/100 cases)in the control group, significantly higher than that of the treated group (62.7%, 64/102 cases). The frequency of E3/4 genotype was 6% (6/100 cases) in the control group, significantly lower than that of the treated group (21.6%, 22/102 cases; both P <0.05). Compared with the control group, the serum levels of TNF-α, IL-6, and hs-CPR were higher in the treated group before treatment ( P <0. 05). In hyperlipidemia patients with E3/4 + E4/4 genotype, the serum level of TNF-α was higher than that of E3I3 genotype (P <0. 05); the serum level of IL-6 was higher than that of E2/E2 +E2/E3 genotype (P<0. 05); the serum level of hs-CRP was higher than that of E2/E2 +E2/E3 and E3/E3 genotype (P<0.05).But there was no statistical difference in the serum levels of TNF-α, IL-6, or hs-CPR between E3/3 and E2/E2 + E2/E3 genotype. After 6-week intervention of Xuezhikang Capsule, the serum levels of TNF-α, IL-6 , and hs-CRP were lower in the treated group (P <0. 05), but the serum levels of TNF-α and IL-6 were still higher than those of the control group (P <0. 05). But there was no statistical difference in the decrement of TNF-α, IL-6, or hsCRP among E2/E2 +E2/E3, E3/E3, or E3/4 +E4/4 genotypes (P>0.05). Conclusions The distribution of ApoE gene polymorphism is different between the hyperlipi-demia patients and the healthy people. Chronic inflammatory reactions exist in hyperlipidemia patients, especially in those with e4 allele. Xuezhikang Capsule showed anti-inflammatory effects, but ApoE gene polymorphism did not affect its effects.关键词
高脂血症/载脂蛋白E/基因多态性/血脂康胶囊/炎症Key words
hyperlipidemia/ apolipoprotein E/ gene polymorphism/ Xuezhikang Capsule/ inflammation引用本文复制引用
蒋卫民,方祝元,朱长乐,唐蜀华..载脂蛋白E基因多态性对血脂康胶囊抑制炎症作用的影响[J].中国中西医结合杂志,2013,33(1):35-39,5.基金项目
江苏省自然基金项目(No.BK2005162) （No.BK2005162）
