中国中西医结合杂志2013，Vol.33Issue(1)：51-55,5.
复方丹参注射液对人羊膜上皮细胞水通道蛋白3表达的影响
Effects of Compound Salvia miltiorrhiza Injection on Aquaporin 3 Expression in Human Amniotic Epithelial Cells
摘要
Abstract
Objective To study the effects of Compound Salvia miltiorrhiza Injection (CSI)on aquaporin 3 (AQP3)expression in human amniotic epithelial cells (hAECs), and to explore its mechanisms for treating oligohydramnios. Methods The hAECs selected from 8 human term pregnancies with oligo-hydramnios and no other complications (as the test group)and 8 human term pregnancies with normal amniotic fluid volume (as the control group)were primarily cultured. The mRNA and protein expressions of AQP3 in hAECs were detected using reverse transcription-polymerase chain reaction and Western blot with various concentrations of CSI (0. 000 , 0. 001 , 0. 010 , 0. 020 , 0. 060 , and 0. 100 mg/mL, respectively)at different time points (0, 6, 12 , 24 , and 48 h, respectively). Results (1) Com pared with the control group, the AQP3 expression was down-regulated in the test group (P<0.05). (2)The AQP3 expression in the two groups reached the peak when the concentration of CSI was 0.010 mg/mL, showing statistical difference when compared with other concentrations (P<0.05). (3)The AQP3 expression reached the peak when 0.010 mg/mL CSI acted for 12 h, showing statistical difference when compared with other concentrations (P <0. 05). (4)The AQP3 expression was up-regulated in the two groups when 0. 010 mg/mL CSI acted for 12 h. But the up-regulated AQP3 expression was more obvious in the test group than in the control group (P<0.05). Conclusions CSI could regulate the AQP3 expression in hAECs. CSI showed more obvious effects on the AQP3 expression in hAECs of oligohydramnios human term pregnancies.关键词
水通道蛋白3/羊水过少/人羊膜上皮细胞/复方丹参注射液Key words
aquaporin3/ oligohydramnios/ human amniotic epithelial cell/ Compound Salvia miltiorrhiza Injection引用本文复制引用
张译文,马小燕,丁盛娣,谢爱兰,朱雪琼..复方丹参注射液对人羊膜上皮细胞水通道蛋白3表达的影响[J].中国中西医结合杂志,2013,33(1):51-55,5.基金项目
浙江省中医药管理局重点项目(No.2010ZZ009) （No.2010ZZ009）
温州市对外合作交流科技计划项目(No.H20100067) （No.H20100067）
