中国中西医结合杂志2013，Vol.33Issue(1)：71-75,5.
脾阳虚证模型大鼠回肠的比较蛋白质组学探析
Comparative Proteomics Analysis of the Ileum of Pi-yang Deficiency Model Rats
摘要
Abstract
Objective Based on proteomics technology, Pi-yang deficiency syndrome (PYDS) correlated differential proteins were screened, thus providing powerful experiment reliance for exploring the essence of PYDS. Methods Totally 36 SD rats of SPF grade were randomly divided into the normal control group ( n =16)and the PYDS group ( n =20). The PYDS model rats were induced by improper diet , overstrain, and administration of yang impairing bitter cold herbs. The total proteins of the ileum were separated and extracted from rats in the PYDS group and the normal control group. The differential protein dots were identified using Delyder? 2D 6. 5 image analysis software by two-dimensional gel electro-phoresis (2-DE)technology. The finger print map of corresponding peptide qualities was obtained by applying MALDI TOF/TOF. The differential proteins were identified using Mascot search library. Results Judged by statistics and fuzzy mathematics, Pi-yang deficiency rat model was successfully established. Eight proteins with differential expressions involving cell skeleton, energy metabolism, and signal trans-duction, and so on were obtained. Of them , there were 4 up-regulated proteins, i.e., desmin, cytokera-tin8 (CK8), pyruvate kinase (PK), and ezrin. Four down-regulated proteins were glyceraldehyde-3-phos-phate dehydrogenase ( GAPDH ), cytokeratin19 (CK19), cytokeratini ( CK1 ), and actin. Conclusion The pathogenesis of PYDS might be slowed energy metabolism rate, reduced energy production, changed structure of ileal villin, and weakened absorbing and digestive functions.关键词
脾阳虚证/比较蛋白质组学/回肠Key words
Pi-yang deficiency syndrome/ comparative proteomics/ the ileum引用本文复制引用
于漫,吕凌,王彩霞,贾连群..脾阳虚证模型大鼠回肠的比较蛋白质组学探析[J].中国中西医结合杂志,2013,33(1):71-75,5.基金项目
国家自然科学基金资助项目(No.30973689) （No.30973689）
