中国中西医结合杂志2013，Vol.33Issue(1)：90-94,5.
还脑益聪方提取物对APP转基因小鼠脑组织Aβ生成相关因子和学习记忆行为的影响
Effects of Huannao Yicong Recipe Extract on the Learning and Memory and Related Factors of Aβ Generation in the Brain of APP Transgenic Mice
摘要
Abstract
Objective To study the effects of Huannao Yicong Recipe (HNYCR) extract on the learning and memory ability, and the expressions of amyloid precursor protein (APP), p-site APP-clea-ving enzyme 1 (BACE1) , presenilin-1 ( PS-1), and beta amyloid protein (Ap) in hippocampus CA1 area of APP transgenic mice, and to explore its mechanisms for treating Alzheimer's disease (AD). Methods Totally 3-month-old APP695V717I transgenic mice were used to establish the AD model in this research. They were randomly divided into the model group, the Donepezil group, the large dose HNYCR extract group, the small dose HNYCR extract group, and the normal control group (C57BL/6J mice), 15 in each group. These animals were gavaged for 4 continuous months. Relevant indicators were detected: Morris water maze test was used to measure the spatial learning and memory ability. The immunohistochemical assay was used to detect the expressions of APP, BACE1 , PS-1 , and Ap. Results The times of crossing the original platform and the swimming time and distance in the fourth quadrant of the 7-month-old APP transgenic mice were significantly reduced in Morris water maze test, when compared with the normal control group (P<0.01).The times of crossing original platform and the swimming time and distance in the fourth quadrant of all treatment groups significantly increased in Morris water maze test, when compared with the model group (P <0. 05). The expressions of APP, BACE1, PS-1, and A(3 in hippocampus CA1 area of 7-month-old model mice increased significantly (P<0.01), when compared with the normal control group. The expressions of APP, BACE1 , PS-1 , and A(3 in each 7-month-old intervention groups were significantly reduced, when compared with the model group ( P <0. 01). Conclusion Early application of HNYCR extract can obviously improve the learning and memory ability of APP transgenic mice that has declined, reduce the expressions of APP, BACE1, PS-1 , and Aβ in the hippocampal CA1 area, reduce the production of Aβ, and slow down the pathological process of brains in APP transgenic mice.关键词
阿尔茨海默病/APP转基因小鼠/学习记忆/β淀粉样蛋白/β位点剪切酶/早老素蛋白-1/还脑益聪方提取物Key words
Alzheimer's disease/APP transgenic mice/ learning and memory/beta amyloid protein /β-site APP cleaving enzyme 1 /presenilin-1 /Huannao Yicong Recipe extract引用本文复制引用
李浩,刘明芳,刘剑刚,刘龙涛,官杰,蔡琳琳,胡佳,魏芸..还脑益聪方提取物对APP转基因小鼠脑组织Aβ生成相关因子和学习记忆行为的影响[J].中国中西医结合杂志,2013,33(1):90-94,5.基金项目
国家自然科学基金资助项目(No.30873338,No.81173383) （No.30873338,No.81173383）
国家科技部重大新药创制项目(No.2009ZX09103-391) （No.2009ZX09103-391）
