中药材2012，Vol.35Issue(9)：1374-1377,4.
麻花秦艽休眠芽的玻璃化超低温保存及植株再生
Cryopreservation and Plantlet Regeneration of Dormant Buds of Gentiana straminea by Vitrification
摘要
Abstract
Objective:To investigate the detailed techniques for cryopreservation of Gentiana straminea dormant buds by vitrification. Methods:Dormant buds as an experimental material, the influence of the different size of dormant buds, preculture and PVS etc. on cryopreservation of Gentiana straminea were studied. Results: The optimal procedures were as follows:10 ～ 11 mm long dormant buds which were cultured on MS medium supplemented with different sucrose concentration (0. 3,0. 5 or 0. 7 mol/L) for 1 day respectively. The buds were immersed in loading solution for 20 min at 20 ℃, and then treated in PVS2 solution for 40 min at 0 ℃ and finally plunged into liquid nitrogen quickly. After 24 hours,the buds were rapidly thawed in a water bath at 40 ℃ for 2 ～ 3 min and washed twice with MS medium supplemented with 1/2 MS liquid medium containing 1. 2 mol/L sucrose. Finally the buds were transferred to regeneration medium( MS + 0. 5 mg/L 6-BA + 0. lmg/L NAA + 3% sucrose + 0. 7% agar), the survival rate was up to 83. 3%. Conclusion:A high-efficiency cryopreservation protocol of Gentiana straminea is set up.关键词
麻花秦艽/休眠芽/超低温保存/玻璃化法Key words
Gentiana straminea Maxim /Dormant buds/Cryopreservalion/Vitrification method分类
医药卫生引用本文复制引用
晋玲,刘进,张延红,朱田田,陈红刚..麻花秦艽休眠芽的玻璃化超低温保存及植株再生[J].中药材,2012,35(9):1374-1377,4.基金项目
甘肃省中医药管理局项目(GZK-2010-Z2) （GZK-2010-Z2）
国家中医药管理局项目(04-C52L23) （04-C52L23）
