中药材2012，Vol.35Issue(9)：1382-1385,4.
土鳖虫多肽的制备及免疫调节作用研究
Study on the Processing Technique of Enzyme Hydrolysis Eupolyphaga sinensis Peptide and Immunoregulatory Effect
摘要
Abstract
Objective:Using papain to prepare polypeptide from Eupolyphaga sinensis, then study the immune function of polypeptide from Eupolyphaga sinensis in vivo. Methods:Used hydrolysis degree as index,pH value,enzyme dosage,thermometer reaction time were optimized. Studied the influence of polyeptide on the mice immune functions through mice immune organs index, phagocytic function and the level of IL-2. Results:The optimum enzymolysis condition was as follows; pH 8. 0,enzyme 1% , temperature 55 ℃,reaction time 4.5 h. In vim test of mice demonstraed that, Eupolyphaga sinensis could elevate index of thymus and spleen, enhance the phagocytic function of macrophage and promote the level of IL-2 in serum. Conclusion; Eupolyphaga sinensis has immunoregulatory effect.关键词
土鳖虫/多肽/免疫调节Key words
Eupolyphage sinensis Walker/Polypeptide/Immunoregulatory分类
医药卫生引用本文复制引用
刘丹,李兴暖,秦仲君,何巍,赵勇..土鳖虫多肽的制备及免疫调节作用研究[J].中药材,2012,35(9):1382-1385,4.基金项目
江西省卫生厅科学基金(No.20092078) （No.20092078）
江西省教育厅青年科学基金(No.GJJ10264) （No.GJJ10264）
