中药材2012，Vol.35Issue(9)：1388-1391,4.
应用RAPD技术对溪黄草基原植物分类鉴定
Identification of Herba Rabdosiae Serrae from Different Plant Resources by RAPD Method
摘要
Abstract
Objective:To identify the different plant resources of Herba Rabdosiae Serrae by using Random Amplified Polymorphic DNA ( RAPD ) Analysis. Methods: The Mini Spin Columns were used to extract the genomic DNA from five different plants of Herba Rabdosiae Serrae. With the DNA extracted from these plants as template,the 85 oligo nucleic acids (10 bp)as random primers,the Polymer Chain Reaction(PCR) was done and the results were analysed by electro-pharoses. Results: 12 primers were selected with polymorphism and 7 of them showed good polymorphism in RAPD map. Conclusion; RAPD method can be used to identify the plant resources of Herba Rabdosiae Serrae.关键词
溪黄草/线纹香茶菜/RAPD/分子标记技术分类
医药卫生引用本文复制引用
莫小路,曾庆钱,黄珊珊,蔡岳文,王玉生,严振..应用RAPD技术对溪黄草基原植物分类鉴定[J].中药材,2012,35(9):1388-1391,4.基金项目
广东省科技厅粤港关键领域重点突破项目(2009A030901011) （2009A030901011）
