中药材2012，Vol.35Issue(9)：1460-1463,4.
葛根芩连汤有效成分不同配伍的肠吸收Caco-2细胞模型研究
Cellular Absorption of the Active Ingredients in Different Compatibilities of Gegenqinlian Decoction in Human Intestinal Caco-2 Cells
摘要
Abstract
Objective:To study the intestinal dynamics characteristics of puerarin and other active ingredients in Gegenqinlian de-coction.Methods:Caco-2 cell monolayer model was used to research the transport of puerarin and baicalin.berberine hydrochloride, liquiritin when they were used in different compatibilities,meanwhile a ultra performance liquid chromato-graphic method was also developed. Results:The concentration of puerarin, baiealin, berberine hydrochloride,liquiritin in cell culture medium was measured and the apparent permeability coefficients ( Papp) as well as percent absorption (% ) from apical side to basolaleral side were calculated. The absorption characteristics of the four components were different when they were used in different compatibilities. Furthermore the Papp sequence of puerarin in specific groups were puerarin-baicalin-berberine-liquiritin group > puerarin-baicalin group > puerarin-ber-berine > puerarin-liquiritin group > single puerarin (P <0. 05). Conclusion:The intestinal absorption dynamics process of the active ingredients in Gegenqinlian decoction changes as the change of prescription compatibilities. And the intestinal absorption characteristics of puerarin is enhanced by the use of other components.关键词
葛根芩连汤/配伍/Caco-2细胞单层模型/表观渗透系数/UPLCKey words
Gegenqinlian decoction/Different compatibilities/Caco-2 cell monolayer model/Permeability coefficients/UPLC分类
医药卫生引用本文复制引用
张伯莎,安叡,王跃,王新宏..葛根芩连汤有效成分不同配伍的肠吸收Caco-2细胞模型研究[J].中药材,2012,35(9):1460-1463,4.基金项目
2011年度上海市教育委员会科研创新面上项目(11YZ69) （11YZ69）
2011年度上海市教育委员会科研创新重点项目(11ZZ110) （11ZZ110）
高等学校博士点专项科研基金(20093107110011) （20093107110011）
