中药材2012，Vol.35Issue(10)：1668-1674,7.
效应面法优化酶法提取大黄总蒽醌类成分的工艺研究
Optimization of Enzymolysis Extraction of Total Anthraquinones from Wild Rhubarb (Rheum palmatum) Via Response Surface Methodology
摘要
Abstract
Objective:To optimize the technology of enzymolysis extraction of total anthraquinones.Methods: Used the total an-thraquinone concentration extracted from rhubarb (Rheum palmatum,L) via enzymolysis as experimental assess index,effect of enzyme dose,enzyme solution temperature,enzyme solution time and pH on total anthraquinone extraction were investigated respectively based on single factor variance analysis.Furthermore,its extraction process was optimized based on response surface methodology.Re-sults:The yield of total anthraquinones demonstrated the top (3.1730 ±0.1023 mg/L) while the enzyme dose was up to 0.12 g,besides,for the enzyme solution time,the total anthraquinones yield exhibited a trend of increase with the increase of enzyme solution time and the top concentration of total anthraquinones was up to 2.1410 ±0.1252 mg/L while it was 48 h; Moreover,the top field of total anthraquinones were up to 2.2777 ± 0.2133 mg/L and 4.1360 ± 0.3877 mg/L while the pH and enzyme solution temperature were up to 4.8 and 50 ℃ in respectively.Finally,the most optimum enzymolysis extraction processing technology via response surface methodology was as follows:pH:4.59,enzyme dose:0.1 g as well as enzyme solution temperature; 45.86 ℃.Conclusion: The condition of extraction processing technology is easily-controlled,simple and lower cost,and the final extraction concentration of total anthraquinone is up to 4.3231 mg/L under the processing technology above.关键词
效应面法/酶法/大黄/总蒽醌Key words
Response surface methodology/Enzymolysis/Rhubarb (Rheum palmatum L. )/Total anthraquinone/Optimization分类
医药卫生引用本文复制引用
周天林,井明博,李东波,焦飞..效应面法优化酶法提取大黄总蒽醌类成分的工艺研究[J].中药材,2012,35(10):1668-1674,7.基金项目
甘肃省庆阳市重大支撑项目(1003NKCM020) （1003NKCM020）
科技部国家科技支撑计划子课题(2006BA106A-04) （2006BA106A-04）
中央高校基本科研业务费项目(GK201002080) （GK201002080）
