中药材2012，Vol.35Issue(11)：1799-1804,6.
天麻中天麻素提取检测方法研究
Research the Extraction and Detection Method of Gastrodin Content in Gastrodia elata
摘要
Abstract
Objective:To improve an extraction and determination method for gastrodin in Gastrodia elata.Methods:On the basis of single factor experiment,used the method of hot water reflux extraction and applied the response surface methodology to determine the best conditions for extracting gastrodin and using HPLC test.Results:Under the boiling water condition,pure water as solvent,the solid-liquid ratio was 1:37,extraction 2 times and extraction time 126 min per times,the content of gastrodin was 0.377%.Comparison with the 2010 edition of the China pharmacopoeia of gastrodin extracted in Gastrodia elata,the result showed the gastrodin content was increased by 2.12 times.Conclusion:This extraction method of gastrodin dissolution rate is high and can reflect the real content of gastrodin of Gastrodia elata .关键词
天麻/天麻素/响应面分析法Key words
Gastrodia elata Bl. /Gastrodin/Extraction/Detection/Response surface methodology分类
医药卫生引用本文复制引用
徐顶巧,周建军,刘益红..天麻中天麻素提取检测方法研究[J].中药材,2012,35(11):1799-1804,6.基金项目
陕西省"13115"科技创新工程项目(2010ZDGC-23) （2010ZDGC-23）
