中药材2012，Vol.35Issue(11)：1805-1808,4.
三七总皂苷对快速老化SAMP8小鼠大脑α分泌酶含量及活性的影响
The Effect of PNS on the Content and Activity of α-Secretase in the Brains of SAMP8 Mice with Alzheimer's Disease
摘要
Abstract
Objective:To explore the effects of PNS on the content and activity of α-secretase in the brains of SAMP8 mice with Alzheimer's disease.Methods:SAMP8 mice were randomly divided into four groups:PNS high-dosage group,PNS low-dosage group,hu-perzine A group and control group.The high-dosage group and low-dosage group were treated with 200 and 100 mg/kg PNS respectively per day and the huperzine A group was treated with 0.3 mg/kg huperzine A per day,all by intragastric administration for 8 consecutive weeks.The same volume of double distilled water was given to the control group.The activity of a-secretase was assayed by direct immu-nofluorescent method( DIF).Western blot was used to detect the content of a-secretase including ADAM9 ,ADAM10 and ADAM17 proteins.Results:The Relative Fluorescence Units (RFU) of PNS high-dosage and low-dosage groups were higher than that of control group (P <0.01).The results of western blot showed that the level of ADAM9 protein expression in PNS high-dosage,low-dosage and huperzine A groups was significantly higher than that of control group (P <0.05) while the levels of ADAM 10 protein expression in PNS high-dosage,low-dosage and huperzine A groups was significantly lower than that of control group(P <0.05) .while level of ADAM17 of huperzine A group was higher than that of control group(P <0.05).Conclusion:PNS can increase activity of a-secretase in the brain of SAMP8 mouse via increasing the level of ADAM9 protein expression.关键词
三七总皂苷/SAMP8/α分泌酶Key words
PNS/SAMP8/α-Secretase分类
医药卫生引用本文复制引用
黄金兰,吴登攀,陆璐,李霏,钟振国..三七总皂苷对快速老化SAMP8小鼠大脑α分泌酶含量及活性的影响[J].中药材,2012,35(11):1805-1808,4.基金项目
国家自然科学基金项目(30960489,81160512) （30960489,81160512）
广西自然科学基金项目(2010GXNSFD013050) （2010GXNSFD013050）
