中药材2012，Vol.35Issue(11)：1817-1820,4.
雄黄亚慢性染毒对未成年大鼠脑组织内Glu和Gln水平的影响
Effect of Subchronic Realgar Exposure on Glu and Gln in Infant Rat Brain
摘要
Abstract
Objective:To study the effect of realgar on Glu and Gin on rat brain tissues.Methods:Forty-eight Wistar rats were divided into 4 groups randomly:control group,low dosage group,moderate dosage group and high dosage group.The treatment groups were treated with realgar by gastric perfusion at a dosage of 0.3 g/kg,0.9 g/kg,2.7 g/kg and the control group ones were orally given the same volume of 0.5% sodium carboxymethylcellulose (CMC-Na) for 6 weeks.The contents of inorganic arsenic,monomethylarsonic acid (MM A) and dimethylarsinic acid (DMA) in brain tissues were measured by hydride generation-atomic absorption (HG-AAS) method.The contents of amino acid neurotransmitters in brain tissues of rats were determined by means of high performance liquid chro-matography with precolumn derivatization.Results:The levels of MMA and DMA in brain increased as the dosage of realgar increased,while the second methylation index declined.Compared with control group,the levels of Glu was significantly decreased in realgar treated group ( P < 0.05 ); Gin also tended to decrease and that of low dosage group was obviously decreased compared with controls.Conclusion:Realgar exposure can cause accumulation of MMA and DMA,declination of second methylation index and the reduction of Glu and Gin in brain tissue.关键词
雄黄/形态砷/谷氨酸/谷氨酰胺Key words
Realgar/Arsenic/Glu /Gln分类
医药卫生引用本文复制引用
畅蓓,霍韬光,张颖花,李维凯,杨卉蕾,姜泓,孙贵范..雄黄亚慢性染毒对未成年大鼠脑组织内Glu和Gln水平的影响[J].中药材,2012,35(11):1817-1820,4.基金项目
国家自然科学基金资助项目(81073144) （81073144）
辽宁省自然科学基金资助项目(20092133) （20092133）
辽宁省教育厅高等学校科研资助项目(L2010708) （L2010708）
