中药材2012，Vol.35Issue(11)：1820-1824,5.
血清药理学研究车前子对成骨细胞功能的影响
Effects of Drug Serum of Plantaginis Semen on Bone Formation Function of Rat Osteoblast in vitro
摘要
Abstract
Objective:To study the influence of Plantaginis Semen on cell proliferation,differentiation and function of rat osteoblasts,and investigate the regulation effects of rat osteoblast epithelial sodium channel(ENaC) on bone formation.Methods:The animal serum was prepared by serum pharmacology means.The cells were got by separating and inducing the SD neonatal rat's skull bone.Cell proliferation and differentiation were evaluated by CCK-8 assay kit and AKP assay kit respectively.Regulation effects on mRNA expression of ENaC and osteogenesis gene were investigated by semi-quantitative PCR.Results:Plantaginis Semen stimulated the osteoblasts proliferation and differentiation,the difference between treatment group and control group had statistical signifieance(P<0.01)in a dose-dependent manner.The effects of Plantaginis Semen serum on α-ENaC gene expression paralleled those on osteogenic gene ( OC,ALP,OP) expression level.Conclusion:Plantaginis Semen stimulates proliferation,differentiation and the mRNA expression of ENaC and osteogenesis gene in rat osteoblasts.Our results suggest that ENaC participate in the effects of Plantaginis Semen serum on osteoblast bone formation.Regulation of ENaC channel expression and function may provide a new clue for research on treatment of osteoporosis with traditional Chinese medi- cine.关键词
车前子/血清药理学/上皮钠离子通道/成骨细胞Key words
Plantaginis Semen/Serum Pharmacology/Epithelial sodium channel/Osteoblast分类
医药卫生引用本文复制引用
陈珺,刘家剑,卢丽,杨国柱,陆幸妍,黄剑,李青南..血清药理学研究车前子对成骨细胞功能的影响[J].中药材,2012,35(11):1820-1824,5.基金项目
2009国家自然科学基金面上项目(30971172) （30971172）
2009广东省科技计划项目(2010B050700022) （2010B050700022）
