中药材2012,Vol.35Issue(12):1925-1928,4.
不同产地桑椹药材HPLC指纹图谱研究
Study on the Fingerprint of Morus alba from Different Habitats by HPLC
摘要
Abstract
Objective: To establish HPLC fingerprint of Morus alba from different habitats by HPLC and provide basis for its quality control. Methods; HPLC analysis was performed on an Agilent XDB C18 Column (4. 6 mm ×250 mm,5 μm) ,gradient eluted composed of acetonitrile and 0. 3 % phosphate acid. The column temperature was set at 35 ℃ and the flow rate was 0. 5 ml/min. The detective wavelength was 290 nm. The HPLC fingerprint for 10 batches of Morus alba was studied on their similarity. Results: There were twelve common peaks in the fingerprint. The similarity of 7 batches was above 0. 9 and the other batches had low similarity. Conclusion: The HPLC fingerprint can be used for quality control of Morus alba with high characteristics and specificity.关键词
桑椹/指纹图谱/高效液相色谱Key words
Morus alba L. / Fingerprint/ HPLC分类
医药卫生引用本文复制引用
陈诚,李洪波,王柳萍,李运容,辛宁..不同产地桑椹药材HPLC指纹图谱研究[J].中药材,2012,35(12):1925-1928,4.基金项目
广西卫生厅医疗卫生重点科研课题(重2010092) (重2010092)
广西中医药管理局科研课题(gzzc1005) (gzzc1005)