中医药学报2012，Vol.40Issue(5)：15-18,4.
HPLC法测定乳香中乙酰乳香酸类成分的含量
Quantitative Analysis of Acetyl Boswellic Acids in Frankincense by HPLC
摘要
Abstract
Objective: In order to provide scientific evidence for overall quality evaluation of Frankincense, a trial to develop HPLC method for quantitative Analysis of three Acetyl Boswellic Acids in Frankincense were carried out. Methods: The HPLC method was performed on Diamonsil C8 column ( 250mm ×4. 6mm, 5μm) , with acetonitrile - 0. 1% phospholic acid( A :80:20; B :70= 30) as the mobile phase; UV detection wavelength 210nm for α and β- acetyl boswellic, 250nm for 11 - keto - β- acetyl boswellic acid;the column temperature was 35℃. Results:The liner ranges of a -acetyl boswellic acid, β- acetyl boswellic acid and 11 - keto - β - acetyl boswellic acid were 0. 099 ～ 9. 9μg(0. 9999 ) , 0.0976- 9. 76μg(0.9999) and 0. 0997 - 9. 97μg(0.9999); the average recoveries were 98. 6% , 101.9% and 98. 25% , and RSD were 1. 38% , 1.49% and 1. 86% ( n = 6) ; the content ranges of three boswellic acids in Boswellia cart-erii were 0. 23% ～2. 41% ,0. 45% ～4. 27% and 0. 35% ～5. 27% respectively. Conclusion:The method is proved to be convenient and reliable, so it can be used for quantitative analysis of acetyl boswelic acids.关键词
乳香/α-乙酰乳香酸/β-乙酰乳香酸/11-羰基-β-乙酰乳香酸/HPLCKey words
Frankincense( Boswellia carterii) / a - acetyl boswellic acid/ ?- acetyl boswellic acid/ 11 - keto -?- a-cetyl boswellic acid/ HPLC分类
医药卫生引用本文复制引用
常允平,夏广萍,韩英梅,张俊艳..HPLC法测定乳香中乙酰乳香酸类成分的含量[J].中医药学报,2012,40(5):15-18,4.基金项目
国家重大新药创制专项(2011ZX09401-009) （2011ZX09401-009）
