中医药学报2012，Vol.40Issue(5)：19-21,3.
山莨菪中乙酰胆碱酯酶抑制剂的分离
Isolation of Acetylcholinesterase Inhibitors from Anisodus Tangutcus
摘要
Abstract
Objective: To isolate acetylcholinesterase inhibitor from total alkaloidal extracted of the roots of Anisodus tangutcus. Method; The root of Anisodus tangutcus were extracted by 95%ethanol and treated with 4 mol ·L-1HCl and 4 mol ·L-1 NaOH , and then extracted by chloroform to obtain total alkaloidal extracts. The bioassay - guided isolation of the AChE inhibitor was executed by TLC bioautographic method , and modified Ellman' s colorimetric method in 96 -well microplates. Three compounds (1 - 3) were obtainded by various column chromatographic methods and their structures were elucidated based on'HNMR,13C - NMR , ESI - MS analysis and compared with literature reports. Result: Three known compounds were isolated and identified as scopolamine, anisodine, atropine, respectively. Their inhibitory rates of 1 - 3 against AchE were 45.28% , 38. 08% and 25. 10% , respectively. Conclusion: Derivatives of hyoscyamine have a weak inhibitory activity to AchE.关键词
山莨菪/乙酰胆碱酯酶抑制剂/总生物碱Key words
Anisodus tangutcus/ Acetylcholinesterase inhibitors/ Total alkaloids分类
医药卫生引用本文复制引用
杨中铎,薛鹏辉..山莨菪中乙酰胆碱酯酶抑制剂的分离[J].中医药学报,2012,40(5):19-21,3.基金项目
国家自然科学基金资助项目(20802031) （20802031）
