中医药学报2012，Vol.40Issue(5)：24-27,4.
姜黄素抗C57BL/6小鼠肺纤维化与CathepsinK相关性研究
Effect of Curcumin on Anti-lung Fibrosis of C57BL/6 Mice and Its Relationship with Cathepsink
摘要
Abstract
Objective ;To investigate the mechanism of curcumin on anti - lung fibrotic effect in bleomycin induced lung fibrosis C57BL/6 mice. Methods; Intratracheal application of bleomycin in C57BL/6 mice was done. Briefly, tracheas of anaesthetized mice were surgically exposed and 0. 025 U of bleomycin diluted in sterile saline or saline only was slowly instilled . The mice were divided into three groups at random; Control, Bleomycin, Bleomycin + Curcumin (200 mg/ ( kg ·d). From the second day after bleomycin instillation, mice were challenged with saline or curcumin (200mg/(kg ·d) ) in 0.5% carboxymethylcellulose via oral gavage and sacrificed at 7 ,14 and 28 days post - treatment followed by determination of lung fibrosis by estimating lung collagen content. In addition, changes in cathepsin K protein and mRNA levels in the lung were determined by immunohistochemistry, western blotting and real time RT - PCR. Results: Curcumin treated mice responded with a lower collagen deposition in their lungs, which was accompanied by a significantly higher lung resistance compared with bleomycin -treated mice. Moreover, Curcumin treated mice responded with a lower degree of lung fibrosis than only bleomycin - treated mice. In addition, Cathepsin K protein levels were strongly increased in curcumin treated mice relative to only bleomycin treated mice( 14d, P =0. 006;7d,P =0. 015;28d, P =0. 027); curcumin also obviously increased the level of Cathepsin K mRNA(7d, P =0.047;14d,P =0.001). Conclusion:Curcumin increases the expression of cathepsin K and reduces lung collagen deposition in response to bleomycin challenge, thereby providing at least partial protection against bleomycin - induced lung fibrosis.关键词
姜黄素/肺纤维化/CathepsinKKey words
Curcumin/Lung fibrosis/Cathepsin K分类
医药卫生引用本文复制引用
黄春芳,李亚东,沈俊辉,崔向清,李彧,王晓波,王继峰,牛建昭..姜黄素抗C57BL/6小鼠肺纤维化与CathepsinK相关性研究[J].中医药学报,2012,40(5):24-27,4.基金项目
科技部国际科技合作项目(2008DFA31970) （2008DFA31970）
国家自然科学基金项目(81173642) （81173642）
