中医药学报2012，Vol.40Issue(5)：27-29,3.
五味子超临界萃取物对Aβ25-35诱导的PC12细胞损伤后生存状态和活力的保护作用
Protective Effects of Supercritical Extraction Obtained from Schisandra Chinensis for the Survival Condition and Vigor of Aβ 25-35 Damaged PC12 Cells
摘要
Abstract
Objective: To study the protective effects of supercritical extraction obtained from Schisandra chinensis for the survival condition and vigor of PC12 cells damaged by Aβ25-35. Methods; The PC cells were cultured in vitro. An AD cell model about cell damage caused by Aβwas established. The survival condition and vigor of PC12 cells were detected by using the MTT and LDH methods. Results: Supercritical extraction of Schisandra Chinensis could reduce the concentration of LDH and increased the activity of mitochondria dehydrogenase of PC12 cells. The amount of living PC12 cells increased. Conclusion; The extent of injury of Aβ25 -35 damaged PC 12 cells can be relieved and the cell membrane can also be protected by using supercritical extraction of Schisandra Chinensis. The extraction is good for improving the survival condition and vigor of PC 12 cells.关键词
五味子超临界萃取物/阿尔茨海默病/PC12细胞/MTT/LDHKey words
Supercritical extraction of Schisandra Chinensis/ Alzheimer's disease/ PC12 cells/ MTT/ LDH分类
医药卫生引用本文复制引用
谢宁,姜媛媛,于淼,周妍妍..五味子超临界萃取物对Aβ25-35诱导的PC12细胞损伤后生存状态和活力的保护作用[J].中医药学报,2012,40(5):27-29,3.基金项目
国家自然科学基金资助项目(30472128) （30472128）
黑龙江省自然科学基金资助项目(D201014) （D201014）
