中医药信息2012，Vol.29Issue(6)：8-10,3.
龙芽楤木皂苷对培养心肌细胞钠通道电流的影响
Effects of Aralosides on Sodium Chanel Current in Cultured Neonatal Rat Cardiomyocytes
摘要
Abstract
Objective; To study the mechanism of inotropic effect of Aralosides (As) by observing the effects of As on sodium chanel current in cultured neonatal rat cardiomyocytes. Methods: Use standard whole - cell patch clamp recording technique, choose moderate cell as researched object. Experiment was divided into two groups,one was control group and the other was drug group. We recorded normal sodium chanel current as control group, observe effects of different concentration As( lμg/ml, 10μg/ml, 100μg/ml) on sodium current in cultured neonatal rat cardiomyocytes. Results;1) As made Ⅰ- Ⅴ curve of current move down,but it didn' t influence built - in character of sodium sodium chanel current Ⅰ- Ⅴ curve. 2) After adding As 1μg/ml, 10μg/ ml,100μg/ml,As 1μg/ml didn't markedly change sodium current peak ,but As 10μg/ml and 100μg/ml, which concentration - dependently increased sodium currents peak, augmented percentage was 29% and 41% in cultured neonatal rat cardiomyocytes. Conclusion: As concentration - dependently increases sodium currents peak, which can cause intracellular sodium ion to increase, myocardium contraction force is enhanced. One mechanism of inotropic effect of As is carried out by increasing sodium current chanel peak.关键词
龙牙楤木皂苷/钠电流/膜片钳/正性肌力Key words
Aralosides/ INa/ Patch clamp/ Positive inotropic分类
医药卫生引用本文复制引用
张雨薇,马丽娟,李秋元,赵春燕,孙世晓..龙芽楤木皂苷对培养心肌细胞钠通道电流的影响[J].中医药信息,2012,29(6):8-10,3.基金项目
吉林省科技厅资助课题(20050922) （20050922）
