郑州大学学报（理学版）2012，Vol.44Issue(3)：97-100,105,5.DOI:10.3969/j.issn/1671-6841.2012.03.024
油菜农杆菌介导转化体系的建立
Establishment of the Transfer System by Agrobacterium-mediated for Napus
摘要
Abstract
The influence of antibiotic concentration, bacteria liquid concentration, infection time , and cultivation time on the explant differentiation of transgenic napus Zhongyou 821 was researched, and separately analysed. The efficient transformation system of the agrobacterium mediated napus was established. The results showed that, the high transforming condition was achieved when 5 -day cotyledons with petiole were used as transformation receptors; the concentration of kanamycin had great influence on transformation , and the critical concentration was 11 mg/L for this experiment selection ; the explant had the highest conversion rate when the OD600 of the bacteria concentration was 0.4 and the time of immersion was 5 min; the best cultivation time was 48 h and beneficial to the integration of the exogenous gene and without excessive proliferation of the agrobacterium; and both 200μmol/L AS and 5-10 mg/L AgNO3 could improve the rate of the conversion.关键词
外植体/转基因油菜/培养基/共培养Key words
explant/ transgenic napus/ medium/ cocultivation分类
生物科学引用本文复制引用
刘品,赵万千,史团省,沙琰琰,张志杰,朱墨..油菜农杆菌介导转化体系的建立[J].郑州大学学报（理学版）,2012,44(3):97-100,105,5.基金项目
国家自然科学基金资助项目,编号30771301 （）
教育部留学回国人员科研启动项目. （）
