组织工程与重建外科杂志2012，Vol.8Issue(6)：311-315,5.DOI:10.3969/j.issn.1673-0364.2012.06.003
内皮祖细胞对糖尿病心肌病大鼠氧化应激作用的研究
Role of Endothelial Progenitor Cells in Diabetic Cardiomyopathy Rats with Oxidative Stress
摘要
Abstract
Objective To study the role of endothelial progenitor cells (Endothelial progenitor cells, EPCs) in streptozotocin induced diabetic cardiorayopathy oxidative stress. Methods Diabetic rat model was induced by intraperitoneal injection of streptozotocin, treatment group was treated with intravenous injection of EPCs cells. Myocardial tissue morphological changes was detected by HE staining. Fasting blood glucose was detected by glucose oxidase method. The content of GSH and MDA in rat serum were detected by kit. The expression level of eNOS and iNOS of myocardial tissue in rats was measured by RT-PCR and Western blot. Results Compared with the control group, myocardial hypertrophy and tissue disorganization were observed in model group. The content of GSH in serum decreased and the content of MDA increased. iNOS in myocardial tissue and eNOS expression also increased. With the treatment of EPCs, the content of GSH picked up somewhat, while MDA, iNOS and eNOS levels dropped, at the same time, myocardial injury was improved. Conclusion EPCs can alleviate streptozotocin induced diabetic cardiomyopathy and its mechanism may be associated with a reduction in oxidative stress and NOS level.关键词
内皮祖细胞/糖尿病心肌病/氧化应激Key words
EPCs/ Diabetic cardiomyopathy/ Oxidative stress分类
医药卫生引用本文复制引用
成永霞,李志强,刘贵波,颜彬,郭素芬,冯玉宽,陈丽丽,曹永,王宏伟,孙立新..内皮祖细胞对糖尿病心肌病大鼠氧化应激作用的研究[J].组织工程与重建外科杂志,2012,8(6):311-315,5.基金项目
黑龙江省青年科学基金项目(QC2010040) （QC2010040）
黑龙江省自然科学基金项目(D2008-83). （D2008-83）
