现代农业科技Issue(21)：96-98,100,4.
香蕉凝集素基因启动子5′远端的分离分析及鉴定
Isolation and Characterization of the far 5′ Promoter of Banana Lectin Gene
摘要
Abstract
In order to obtain the higher BanLec active promoter, a length of 330 bp fragment was isolated by using chromosomal walking based on the previous banana Lectin gene promoter sequence of 670 bp.A full length of 1 000 bp Banana Lectin gene promoter has been obtained.The results of bioinformatical softwares analysis such as Promoter predictions and Plant CARE showed that there was more tissue-specific expression controlling elements compared with the 670 bp promoter. A plant expression vector contained 1 000 bp BanLec promoter was constructed and transformed to banana roots,leaves and fruits by paficle bombardment.The vectors of which contained 35S promoter and the 670 bp length BanLec's promoter were used as control.The results of transient expression of GUS and GFPshowed that this 1 000 bp BanLec promoter was a fruit-specific promoter,and had a higher activity than 670 bp promoter and CaMV 35S promoter.关键词
香蕉凝集素基因/启动子/果实特异表达Key words
banana leetin gene/promoter/fruit-specific expressed分类
农业科技引用本文复制引用
张建斌,金志强,王纪,刘菊华,贾彩红,徐碧玉..香蕉凝集素基因启动子5′远端的分离分析及鉴定[J].现代农业科技,2012,(21):96-98,100,4.基金项目
“十二五”农村领域国家科技计划 （）
海南省自然科学基金项目 （）
中央级公益性科研院所基本科研业务费专项资金资助项目 （）
海南省重点科技计划（ZDXM20120024）. （）
