大麦与谷类科学Issue(4)：5-9,5.
IT-ISJ标记及其在大麦遗传多样性研究中的应用
IT-ISJ Markers and Its Application to the Genetic Diversity in Barley
摘要
Abstract
IT-ISJ(Intron-targeted intron-exon splice junction),a new molecular marker,the molecular polymorphism of 34 cultivated hulless barley cultivars(lines) from the Qinghai-Tibet Plateau of China,the Europe and the North America,which was reported firstly in this study.The result showed that,20 primer combinations produced a total of 87 clear bands with an average of 4.35 bands per primer pair,and which 81bands(93.10%) were polymorphic.134 alleles,ranged from 2 to 11,were amplified with an average of 6.7 alleles per primer pair.Genetic similarities ranged from 0.437 to 0.931,with an average of 0.743.The average genetic distance(0.257) was lower among accessions and average genetic diversity(0.59),so the result indicated that the genetic basis is narrower among accessions.Based on the results,IT-ISJ marker could be effectively used in researching genetic diversities,cultivar identifications,construction of genetic map and molecular marker-assisted selection breeding.Therefore,it is very essential that the excellent hulless barley genetic resources should be extensively discovered and excavated to widen the genetic basis of hulless barley genetic resources and increase the efficiency in hulless barley breeding.关键词
大麦/青稞/IT-ISJ/遗传多样性Key words
Barley/Hulless Barley/IT-ISJ Marker/Genetic Diversity分类
农业科技引用本文复制引用
余春磊,刘家娴,齐国昌,罗小娇,刘新春,冯宗云..IT-ISJ标记及其在大麦遗传多样性研究中的应用[J].大麦与谷类科学,2012,(4):5-9,5.基金项目
现代农业产业技术体系建设基金 （）
四川省教育厅重点项目 （）
四川农业大学科技成果转化专项基金 （）
